Hybrid graphite film–carbon nanotube platform for enzyme immobilization and protection

Kondyurin, A., Levchenko, I., Han, Z. J., Yick, S., Mai-Prochnow, A., Fang, J., Ostrikov, K., & Bilek, M. M. M. (2013) Hybrid graphite film–carbon nanotube platform for enzyme immobilization and protection. Carbon, 65, pp. 287-295.

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A novel platform consisting of a multilayered substrate, activated graphite-like carbon film, and dense forest of long, vertically-aligned multiwall carbon nanotubes grown by the chemical vapor deposition is designed, fabricated, and tested for covalent immobilization of enzymatic biocatalysts with the aim of protecting them from shear forces and microbial attacks present in bioreactors. The covalent bonding ensures enzyme retention in a flow, while the dense nanotube forest may serve as a protection of the enzymes from microbial attack without impeding the flow of reactants and products. This platform was demonstrated for the two reference enzymes, horseradish peroxidase and catalase, which were immobilized without degrading their biological activity. This combination of an activated carbon layer for an efficient immobilization of biocatalysts with a protective layer of inert carbon nanotubes could dramatically improve the efficiency and longevity of enzymatic bio-catalysis employed in a large variety of advanced biotechnological processes.

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18 citations in Scopus
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18 citations in Web of Science®

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ID Code: 73575
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/j.carbon.2013.08.028
ISSN: 0008-6223
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 09 Jul 2014 01:25
Last Modified: 10 Jul 2014 02:09

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