JK1 (FAM134B) represses cell migration in colon cancer : a functional study of a novel gene

Kasem, Kais, Sullivan, Emily, Gopalan, Vinod, Salajegheh, Ali, Smith, Robert A., & Lam, Alfred King-Yin (2014) JK1 (FAM134B) represses cell migration in colon cancer : a functional study of a novel gene. Experimental and Molecular Pathology, 97(1), pp. 99-104.

View at publisher

Abstract

Background

JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model.

Methods

Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line.

Results

JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates.

Conclusions

JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.

Impact and interest:

3 citations in Scopus
Search Google Scholar™
3 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 74480
Item Type: Journal Article
Refereed: Yes
Additional Information: Kasem, Kais
Sullivan, Emily
Gopalan, Vinod
Salajegheh, Ali
Smith, Robert A
Lam, Alfred K-Y
Exp Mol Pathol. 2014 Jun 10;97(1):99-104. doi: 10.1016/j.yexmp.2014.06.002.
Additional URLs:
Keywords: Colon cancer, FAM134B, JK1, Migration
DOI: 10.1016/j.yexmp.2014.06.002
ISSN: 1096-0945
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 Elsevier
Deposited On: 30 Jul 2014 01:10
Last Modified: 15 Jul 2015 03:16

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page