JK1 (FAM134B) represses cell migration in colon cancer : a functional study of a novel gene
Kasem, Kais, Sullivan, Emily, Gopalan, Vinod, Salajegheh, Ali, Smith, Robert A., & Lam, Alfred King-Yin (2014) JK1 (FAM134B) represses cell migration in colon cancer : a functional study of a novel gene. Experimental and Molecular Pathology, 97(1), pp. 99-104.
JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model.
Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line.
JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates.
JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
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|Item Type:||Journal Article|
|Additional Information:||Kasem, Kais
Smith, Robert A
Lam, Alfred K-Y
Exp Mol Pathol. 2014 Jun 10;97(1):99-104. doi: 10.1016/j.yexmp.2014.06.002.
|Keywords:||Colon cancer, FAM134B, JK1, Migration|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2014 Elsevier|
|Deposited On:||30 Jul 2014 01:10|
|Last Modified:||15 Jul 2015 03:16|
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