The roles of JK-1 (FAM134B) expressions in colorectal cancer

Kasem, Kais, Gopalan, Vinod, Salajegheh, Ali, Lu, Cu-Tai, Smith, Robert A., & Lam, Alfred King-Yin (2014) The roles of JK-1 (FAM134B) expressions in colorectal cancer. Experimental Cell Research, 326(1), pp. 166-173.

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Abstract

The aims of the present study are to investigate the clinicopathological correlations of JK-1(FAM134B) expression and its relationship to carcinogenesis in a colorectal adenoma-adenocarcinoma model. JK-1(FAM134B) protein expression was studied in a colon cancer cell line by Western blot and immunocytochemistry. JK-1(FAM134B) expression profiles at mRNA and protein levels were investigated in cancer tissues from 236 patients with colorectal adenocarcinoma and 32 patients with colorectal adenoma using real-time polymerase chain reaction and immunohistochemistry. The findings were then correlated with the clinicopathological features of these tumours. JK-1(FAM134B) protein was demonstrated in the colon cancer cells by Western blot. The protein was located in the nuclei of the tumour cells at both cellular and tissue levels. In colorectal adenocarcinomas, lower levels of JK-1(FAM134B) protein expression were associated with younger age (p=0.032), larger tumour size (p=0.004), advanced cancer stages (p=0.016) and higher rates of cancer recurrence (p=0.04). Also, lower levels of JK-1(FAM134B) mRNA expression were associated with advanced cancer stages (p=0.02) and presence of lymphovascular invasion (p=0.014). Higher JK-1(FAM134B) mRNA and protein expression levels were identified in adenomas and non-neoplastic mucosae, compared to carcinomas (p=0.005). To conclude, JK-1(FAM134B) mRNA expression and JK1 (FAM134B) protein levels varied with the different stages of progression of colorectal tumours. The expression levels of the gene were associated with clinicopathological features in patients with colorectal adenocarcinoma suggesting that JK-1(FAM134B) gene has roles in controlling some steps in the development of the invasive phenotypes from colorectal adenoma to early staged as well as advanced staged colorectal adenocarcinomas.

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ID Code: 74494
Item Type: Journal Article
Refereed: Yes
Additional Information: Kasem, Kais
Gopalan, Vinod
Salajegheh, Ali
Lu, Cu-Tai
Smith, Robert A
Lam, Alfred K-Y
Exp Cell Res. 2014 Aug 1;326(1):166-173. doi: 10.1016/j.yexcr.2014.06.013. Epub 2014 Jun 25.
Keywords: Adenocarcinoma, Adenoma, Colorectal cancer, Expression, FAM134B, JK-1
DOI: 10.1016/j.yexcr.2014.06.013
ISSN: 0014-4827
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 Elsevier
Deposited On: 30 Jul 2014 00:41
Last Modified: 31 Jul 2014 00:14

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