Regulation of microRNA-1288 in colorectal cancer : altered expression and its clinicopathological significance
Gopalan, Vinod, Pillai, Suja, Ebrahimi, Faeza, Salajegheh, Ali, Lam, Tommy C., Le, Tran K., Langsford, Nicole, Ho, Yik-Hong, Smith, Robert A., & Lam, Alfred King-Yin (2014) Regulation of microRNA-1288 in colorectal cancer : altered expression and its clinicopathological significance. Molecular Carcinogenesis, 53 (S1), E36-E44.
We aim to examine the miR-1288 expression in cancer cell lines and a large cohort of patients with colorectal cancer. Two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The miRNA expressions of miR-1288 were tested on these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). An exogenous miR-1288 (mimic) was used to detect cell proliferation and cell cycle changes in SW480 using MTT calorimetric assay and flow cytometry, respectively. In addition, tissues from 122 patients with surgical resection of colorectum (82 adenocarcinomas, 20 adenomas, and 20 non-neoplastic tissues) were tested for miR-1288 expression by qRT-PCR. The colon cancer cell lines showed reduced expression of miR-1288 compared to normal colonic epithelial cell line. Over expression of miR-1288 in SW480 cell line showed increased cell proliferation and increased G2-M phase cells. In tissues, reduced miR-1288 expression was noted in majority of colorectal adenocarcinoma compared to colorectal adenoma and non-neoplastic tissues. Reduced or absent expression of miR-1288 was noted in 76% (n = 62/82) of the cancers. The expression levels of miR-1288 were higher in distal colorectal adenocarcinomas (P = 0.013) and in cancers of lower T staging (P = 0.033). To conclude, alternation of miR-1288 expression is important in the progression of colorectal cancer. The differential regulation of miR-1288 was found to be related to cancer location and pathological staging in colorectal cancers.
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|Item Type:||Journal Article|
|Additional Information:||Special Issue : Mechanisms and Targets for Prevention and Treatment of Colorectal Cancer|
|Keywords:||Adenocarcinoma/ genetics/mortality/secondary, Adenoma/ genetics/mortality/pathology, Adult, Aged, Aged, 80 and over, Apoptosis, Blotting, Western, Cell Proliferation, Colorectal Neoplasms/ genetics/mortality/pathology, Female, Flow Cytometry, Follow-Up Studies, Gene Expression Regulation, Neoplastic, Humans, Immunoenzyme Techniques, Lymphatic Metastasis, Male, MicroRNAs/ genetics, Middle Aged, Neoplasm Grading, Neoplasm Recurrence, Local/drug therapy/pathology, Neoplasm Staging, Prognosis, RNA, Messenger/genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Survival Rate, Tumor Cells, Cultured|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Therapy (excl. Chemotherapy and Radiation Therapy) (111204)
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2014 John Wiley & Sons|
|Deposited On:||03 Aug 2014 22:27|
|Last Modified:||05 Aug 2014 00:34|
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