Single nucleotide polymorphisms and mRNA expression of VEGF-A in papillary thyroid carcinoma: Potential markers for aggressive phenotypes

Salajegheh, Ali, Smith, Robert A., Kasem, Kais, Gopalan, Vinod, Nassiri, Mohammad, Williams, Richard, & Lam, Alfred King-Yin (2011) Single nucleotide polymorphisms and mRNA expression of VEGF-A in papillary thyroid carcinoma: Potential markers for aggressive phenotypes. European Journal of Surgical Oncology, 37(1), pp. 93-99.

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Polymorphisms of the VEGF gene are known to affect the biological behaviour of cancers but have seldom been studied in thyroid cancer. The aim of the current study is to evaluate the prevalence and relevance of VEGF-A polymorphisms and mRNA expression in papillary thyroid carcinoma (PTC).


Genomic DNA and total RNA were isolated from paraffin-embedded tissue from 91 PTC (51 conventional PTC and 40 follicular variant) and 78 control thyroid tissues. Three DNA polymorphisms (+936C > T, +405C > G and -141A > C) in the 3' and 5' untranslated region (3'-UTR, 5'-UTR) of VEGF-A were studied using PCR and RFLP. Also, the mRNA expression of VEGF-A in these tissues was studied by real-time PCR.


Distribution of polymorphisms in the 5'-UTR (VEGF-VEGF -141A > C and +405C > G) and 3'-UTR (VEGF +936C > T) were all significantly different in PTC and benign thyroid tissue (p = 0.0001, 0.001 and 0.028 respectively). The VEGF -141 C allele was more common in PTC with lymph node metastases (p = 0.026). VEGF + 405 Galleles andVEGF +936 CC genotype were more common in PTC of advanced pathological staging (p = 0.018 and 0.017 respectively). Also, increased expression of VEGF-A mRNA was noted in PTC compared to control (p = 0.009). Within the group of patients with conventional PTC, those with lymph nodal metastases had a higher level of VEGF-A mRNA expression than other patients (p = 0.0003).


These findings suggest that VEGF polymorphisms and mRNA expression may predict the aggressiveness behaviour of thyroid cancer.

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ID Code: 74628
Item Type: Journal Article
Refereed: Yes
Additional Information: Salajegheh, A Smith, R A Kasem, K Gopalan, V Nassiri, M R William, R Lam, A K Y Research Support, Non-U.S. Gov't England Eur J Surg Oncol. 2011 Jan;37(1):93-9. doi: 10.1016/j.ejso.2010.10.010. Epub 2010 Nov 18.
Additional URLs:
Keywords: Adenocarcinoma, Papillary/ genetics/metabolism, Adult, Female, Humans, Male, Middle Aged, Phenotype, Polymorphism, Single Nucleotide, RNA, Messenger/biosynthesis, Thyroid Neoplasms/ genetics/metabolism, Tumor Markers, Biological/ biosynthesis/genetics, Vascular Endothelial Growth Factor A/ biosynthesis/genetics, Papillary thyroid carcinoma, VEGF-A, Polymorphism, SNP, mRNA expression
DOI: 10.1016/j.ejso.2010.10.010
ISSN: 1532-2157
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2011 Elsevier
Copyright Statement: This is the author’s version of a work that was accepted for publication in European Journal of Surgical Oncology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Surgical Oncology, [VOL 37, ISSUE 1, (2011)] DOI: 10.1016/j.ejso.2010.10.010
Deposited On: 03 Aug 2014 22:49
Last Modified: 05 Aug 2014 01:14

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