Altered JS-2 expression in colorectal cancers and its clinical pathological relevance
Lam, Alfred King-Yin, Gopalan, Vinod, Nassiri, Mohammad, Kasim, Kais, Dissanayake, Jayampathy, Tang, Johnny Chuek-on, & Smith, Robert A. (2011) Altered JS-2 expression in colorectal cancers and its clinical pathological relevance. Molecular Oncology, 5(5), pp. 475-481.
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JS-2 is a novel gene located at 5p15.2 and originally detected in primary oesophageal cancer. There is no study on the role of JS-2 in colorectal cancer. The aim of this study is to determine the gene copy number and expression of JS-2 in a large cohort of patients with colorectal tumours and correlate these to the clinicopathological features of the cancer patients. We evaluated the DNA copy number and mRNA expression of JS-2 in 176 colorectal tissues (116 adenocarcinomas, 30 adenomas and 30 non-neoplastic tissues) using real-time polymerase chain reaction. JS-2 expression was also evaluated in two colorectal cancer cell lines and a benign colorectal cell line. JS-2 amplification was noted in 35% of the colorectal adenocarcinomas. Significant differences in relative expression levels for JS-2 mRNA between different colorectal tissues were noted (p = 0.05). Distal colorectal adenocarcinoma had significantly higher copy number than proximal adenocarcinoma (p = 0.005). The relative expression level of JS-2 was different between colonic and rectal adenocarcinoma (p = 0.007). Mucinous adenocarcinoma showed higher JS-2 expression than non-mucinous adenocarcinoma (p = 0.02). Early T-stage cancers appear to have higher JS-2 copy number and lower expression of JS-2 mRNA than later stage cancers (p = 0.001 and 0.03 respectively). Colorectal cancer cell lines showed lower expression of JS-2 than the benign colorectal cell line. JS-2 copy number change and expression were shown for the first time to be altered in the carcinogenesis of colorectal cancer. In addition, genetic alteration of JS-2 was found to be related to location, pathological subtypes and staging of colorectal cancer.
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|Item Type:||Journal Article|
|Additional Information:||Lam, Alfred King-Yin
Nassiri, Mohammad Reza
Tang, Johnny Chuek-On
Smith, Robert Anthony
Research Support, Non-U.S. Gov't
Mol Oncol. 2011 Oct;5(5):475-81. doi: 10.1016/j.molonc.2011.06.003. Epub 2011 Jul 12.
|Keywords:||Adult, Aged, Colorectum, Mucinous, Cell Line, Tumor, Colorectal Neoplasms/ genetics/ pathology, Female, Gene Dosage/genetics, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Neoplasm Proteins/ genetics/metabolism, RNA, Messenger/genetics/metabolism, Adenocarcinoma, Adenoma, JS-2 gene|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Genetics (111203)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2011 Elsevier|
|Copyright Statement:||This is the author’s version of a work that was accepted for publication in Molecular Oncology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular Oncology, [VOL 5, ISSUE 5, (2011)] DOI: 10.1016/j.molonc.2011.06.003|
|Deposited On:||03 Aug 2014 22:55|
|Last Modified:||05 Aug 2014 01:06|
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