Fos-tau-LacZ mice expose light-activated pathways in the visual system

Greferath, Ursula, Nag, Nupur, Zele, Andrew J., Bui, Bang V., Wilson, Yvette, Vingrys, Algis J., & Murphy, Mark (2004) Fos-tau-LacZ mice expose light-activated pathways in the visual system. NeuroImage, 23(3), pp. 1027-1038.

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Abstract

We have employed fos-tau-LacZ (FTL) transgenic mice to examine functional activation in the visual areas of the nervous system. The FTL mice express the marker gene lacZ in neurons and their processes following many different stimuli, and allow the imaging of activation from the level of the entire brain surface through individual neurons and their projections. Analysis of FTL expression in the retinas of mice following diurnal exposure to light shows that bipolar cells, specific classes of amacrine cells, ganglion cells, and a dense network of processes in the inner plexiform layer are functionally activated. In animals deprived of light, there is almost no activity in the retina. In the lateral geniculate nucleus (LGN), light exposure appears responsible for FTL expression in dorsal nuclei, but not for expression in the ventral nuclei or the intergeniculate leaflet. In the superficial layers of the superior colliculus, FTL expression is highly dependent on light exposure. Similarly, light exposure is required for FTL expression in primary visual cortex (area 17), but some expression remains in area 18 of dark-adapted animals. Finally, using mice with one or both eyes missing, we have determined which parts of the visual system are dependent on the presence of a functional connectivity from the eye. These data demonstrate the usefulness of the FTL mice to map functional activation within the entire visual system. Furthermore, we can capture visual activation in a conscious animal. Our findings give an insight into the architecture of activity within the retina and throughout the visual system.

Impact and interest:

10 citations in Scopus
10 citations in Web of Science®
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ID Code: 7477
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal’s website (see link) or contact the author. Author contact details: andrew.zele@qut.edu.au
DOI: 10.1016/j.neuroimage.2004.06.044
ISSN: 1053-8119
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2004 Elsevier
Deposited On: 04 May 2007 00:00
Last Modified: 12 Nov 2012 23:11

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