Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination

Dichtl, Bernhard, Blank, Diana, Sadowski, Martin, Huebner, Wolfgang, Weiser, Stefan, & Keller, Walter (2002) Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. The EMBO Journal, 21(15), pp. 4125-4135.

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Abstract

RNA polymerase II (pol II) transcription termination requires co‐transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA‐binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted β‐propeller‐forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C‐terminal domain (CTD) of pol II in vitro and in a two‐hybrid test in vivo. Furthermore, transcriptional run‐on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3′‐end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination.

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ID Code: 74850
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months
DOI: 10.1093/emboj/cdf390
ISSN: 1460-2075
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 European Molecular Biology Organization
Deposited On: 10 Aug 2014 22:38
Last Modified: 03 Feb 2015 00:45

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