Structure and assembly of immature HIV

Briggs, John A. G., Riches, James D., Glass, B., Bartonova, V., Zanetti, Giulia, & Kräusslich, Hans-Georg (2009) Structure and assembly of immature HIV. Proceedings of the National Academy of Sciences, 106(27), pp. 11090-11095.

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The major structural components of HIV are synthesized as a 55-kDa polyprotein, Gag. Particle formation is driven by the self-assembly of Gag into a curved hexameric lattice, the structure of which is poorly understood. We used cryoelectron tomography and contrast-transfer-function corrected subtomogram averaging to study the structure of the assembled immature Gag lattice to approximate to 17-angstrom resolution. Gag is arranged in the immature virus as a single, continuous, but incomplete hexameric lattice whose curvature is mediated without a requirement for pentameric defects. The resolution of the structure allows positioning of individual protein domains. High-resolution crystal structures were fitted into the reconstruction to locate protein-protein interfaces involved in Gag assembly, and to identify the structural transformations associated with virus maturation. The results of this study suggest a concept for the formation of nonsymmetrical enveloped viruses of variable sizes.

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191 citations in Scopus
176 citations in Web of Science®
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ID Code: 74985
Item Type: Journal Article
Refereed: Yes
Keywords: cryoelectron tomography, virus assembly
DOI: 10.1073/pnas.0903535106
ISSN: 0027-8424
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Structural Biology (incl. Macromolecular Modelling) (060112)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Virology (060506)
Divisions: Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2009 National Academy of Sciences
Deposited On: 12 Aug 2014 00:10
Last Modified: 22 Jun 2017 07:01

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