Muscle gene electrotransfer is increased by the antioxidant tempol in mice

Markelc, B., Tevz, G., Cemazar, M., Kranjc, S., Lavrencak, J., Zegura, B., Teissie, J., & Sersa, G. (2012) Muscle gene electrotransfer is increased by the antioxidant tempol in mice. Gene Therapy, 19(3), pp. 312-320.

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Abstract

Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore, investigated. We demonstrate in the study that during EP of C2C12 myoblasts, ROS are generated on the surface of the cells, which do not induce long-term genomic DNA damage. Plasmid DNA for transfection (pEGFP-N1), which is present outside the cells during EP, neutralizes the generated ROS. The ROS generation is proportional to the amplitude of the electric pulses and can be scavenged by antioxidants, such as vitamin C or tempol. When antioxidants were used during gene electrotransfer, the transfection efficiency of C2C12 myoblasts was statistically significantly increased 1.6-fold with tempol. Also in vivo, the transfection efficiency of M. tibialis cranialis in mice was statistically significantly increased 1.4-fold by tempol. The study indicates that ROS are generated on cells during EP and can be scavenged by antioxidants. Specifically, tempol can be used to improve gene electrotransfer into the muscle and possibly also to other tissues.

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ID Code: 75280
Item Type: Journal Article
Refereed: Yes
DOI: 10.1038/gt.2011.97
ISSN: 1476-5462
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2012 Macmillan Publishers Limited
Deposited On: 19 Aug 2014 22:40
Last Modified: 21 Aug 2014 00:33

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