Comparative analysis of the Metarhizium anisopliae secretome in response to exposure to the greyback cane grub and grub cuticles

Manalil, Nirupama Shoby, Te’o, Junior, Braithwaite, K.S., Brumbley, S., Samson, P., & Helena Nevalainen, K.M. (2010) Comparative analysis of the Metarhizium anisopliae secretome in response to exposure to the greyback cane grub and grub cuticles. Fungal Biology, 114(8), pp. 637-645.

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Abstract

Metarhizium anisopliae is a well-characterized biocontrol agent of a wide range of insects including cane grubs. In this study, a two-dimensional (2D) electrophoresis was used to display secreted proteins of M. anisopliae strain FI-1045 growing on the whole greyback cane grubs and their isolated cuticles. Hydrolytic enzymes secreted by M. anisopliae play a key role in insect cuticle-degradation and initiation of the infection process. We have identified all the 101 protein spots displayed by cross-species identification (CSI) from the fungal kingdom. Among the identified proteins were 64-kDa serine carboxypeptidase, 1,3 beta-exoglucanase, Dynamin GTPase, THZ kinase, calcineurin like phosphoesterase, and phosphatidylinositol kinase secreted by M. ansiopliae (FI-1045) in response to exposure to the greyback cane grubs and their isolated cuticles. These proteins have not been previously identified from the culture supernatant of M. anisopliae during infection. To our knowledge, this the first proteomic map established to study the extracellular proteins secreted by M. ansiopliae (FI-1045) during infection of greyback cane grubs and its cuticles.

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4 citations in Scopus
4 citations in Web of Science®
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ID Code: 75554
Item Type: Journal Article
Refereed: Yes
Keywords: Electrophoresis, Greyback Cane Grub, Hydrolytic Enzymes, Metarhizium, Secretory Proteins
DOI: 10.1016/j.funbio.2010.05.005
ISSN: 1878-6146
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2010 The British Mycological Society
Deposited On: 25 Aug 2014 23:29
Last Modified: 26 Aug 2014 23:56

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