Biolistic transformation of elite indica rice (Oryza sativa L.) cultivars through semi-solid and liquid medium selection systems

Khanna, Harjeet, Raina, S. K., Srinivasulu, , & Kumar, K. (1997) Biolistic transformation of elite indica rice (Oryza sativa L.) cultivars through semi-solid and liquid medium selection systems. Journal of Plant Biochemistry and Biotechnology, 6(2), pp. 75-80.

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Following microprojectile mediated delivery of a plasmid construct (pAHC-25) encoding bar (bialophos resistance) gene into five-day-old scutellar calli derived from mature embryos, the effectiveness of selection procedure for bar-gene expressing tissue was compared for two indica rice cultivars (IR-64 and Karnal Local). While IR-64 transformants could be selected through the generally used semi-solid selection medium, the same procedure was not effective in the basmati cultivar Karnal Local. In the latter case, while lower concentrations (2–4 mg 1−1) of the selective agent phosphinothricin (PPT) yielded only escapes, higher concentrations (6–8 mg l−1) inhibited proliferation of transformed as well as untransformed sectors. For Karnal Local, a liquid medium based selection system was successfully utilized for recovering transformed sectors and, eventually, regenerants. The study demonstrates the generation of transformants of two elite indica cultivars using the environment-independent system of mature embryos from seeds.

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11 citations in Scopus
7 citations in Web of Science®
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ID Code: 75607
Item Type: Journal Article
Refereed: Yes
Keywords: Oryza sativa, basmati rice, IR-64, transformation, selection
DOI: 10.1007/BF03263014
ISSN: 0974-1275
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Deposited On: 28 Aug 2014 00:11
Last Modified: 22 Jun 2017 11:01

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