Biolistic transformation of elite indica rice (Oryza sativa L.) cultivars through semi-solid and liquid medium selection systems
Khanna, Harjeet, Raina, S. K., Srinivasulu, , & Kumar, K. (1997) Biolistic transformation of elite indica rice (Oryza sativa L.) cultivars through semi-solid and liquid medium selection systems. Journal of Plant Biochemistry and Biotechnology, 6(2), pp. 75-80.
Following microprojectile mediated delivery of a plasmid construct (pAHC-25) encoding bar (bialophos resistance) gene into five-day-old scutellar calli derived from mature embryos, the effectiveness of selection procedure for bar-gene expressing tissue was compared for two indica rice cultivars (IR-64 and Karnal Local). While IR-64 transformants could be selected through the generally used semi-solid selection medium, the same procedure was not effective in the basmati cultivar Karnal Local. In the latter case, while lower concentrations (2–4 mg 1−1) of the selective agent phosphinothricin (PPT) yielded only escapes, higher concentrations (6–8 mg l−1) inhibited proliferation of transformed as well as untransformed sectors. For Karnal Local, a liquid medium based selection system was successfully utilized for recovering transformed sectors and, eventually, regenerants. The study demonstrates the generation of transformants of two elite indica cultivars using the environment-independent system of mature embryos from seeds.
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|Item Type:||Journal Article|
|Keywords:||Oryza sativa, basmati rice, IR-64, transformation, selection|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities|
|Deposited On:||28 Aug 2014 00:11|
|Last Modified:||28 Aug 2014 00:11|
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