Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

Jongbloed, Sarah L., Kassianos, Andrew J., McDonald, Kylie J., Clark, Georgina J., Ju, Xinsheng, Angel, Catherine E., Chen, Chun-Jen J., Dunbar, P. Rod, Wadley, Robert B., Jeet, Varinder, Vulink, Annelie J.E., Hart, Derek N.J., & Radford, Kristen J. (2010) Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. Journal of Experimental Medicine, 207(6), pp. 1247-1260.

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The characterization of human dendritic cell (DC) subsets is essential for the design of new vaccines. We report the first detailed functional analysis of the human CD141(+) DC subset. CD141(+) DCs are found in human lymph nodes, bone marrow, tonsil, and blood, and the latter proved to be the best source of highly purified cells for functional analysis. They are characterized by high expression of toll-like receptor 3, production of IL-12p70 and IFN-beta, and superior capacity to induce T helper 1 cell responses, when compared with the more commonly studied CD1c(+) DC subset. Polyinosine-polycytidylic acid (poly I:C)-activated CD141(+) DCs have a superior capacity to cross-present soluble protein antigen (Ag) to CD8(+) cytotoxic T lymphocytes than poly I:C-activated CD1c(+) DCs. Importantly, CD141(+) DCs, but not CD1c(+) DCs, were endowed with the capacity to cross-present viral Ag after their uptake of necrotic virus-infected cells. These findings establish the CD141(+) DC subset as an important functionally distinct human DC subtype with characteristics similar to those of the mouse CD8 alpha(+) DC subset. The data demonstrate a role for CD141(+) DCs in the induction of cytotoxic T lymphocyte responses and suggest that they may be the most relevant targets for vaccination against cancers, viruses, and other pathogens.

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ID Code: 75622
Item Type: Journal Article
Refereed: Yes
Additional Information: 608AV
Times Cited:237
Cited References Count:67
Keywords: toll-like receptor-3, c-type lectin, in-vivo, t-cells, physiological stimuli, immune-response, ifn-gamma, cd8(+), blood, cd8-alpha(+)
DOI: 10.1084/jem.20092140
ISSN: 0022-1007
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2010 Jongbloed et al.
Copyright Statement: This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at
Deposited On: 22 Jan 2015 01:09
Last Modified: 06 Feb 2015 01:35

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