Fingerprinting of Campylobacter Jejuni by Using Resolution-Optimised Binary Gene Targets Derived From Comparative Genome Hybridization Studies
Price, Erin, Huygens, Flavia, & Giffard, Philip (2006) Fingerprinting of Campylobacter Jejuni by Using Resolution-Optimised Binary Gene Targets Derived From Comparative Genome Hybridization Studies. Applied and Environmental Microbiology, 72(12), pp. 7793-7803.
Abstract
A novel method for genotyping the clustered regularly interspaced short palindromic repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods, and emphasizes the potential of HRM for genotyping short sequence repeats in other species.
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| ID Code: | 7677 |
|---|---|
| Item Type: | Journal Article |
| Additional Information: | For more information, please refer to the journal’s website (see link) or contact the author Flavia Huygens. Author contact details: f.huygens@qut.edu.au |
| Additional URLs: | |
| Keywords: | Campylobacter, Genotyping, Binary Typing, Real-time PCR |
| DOI: | 10.1128/AEM.01338-06 |
| ISSN: | 0099-2240 |
| Subjects: | Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Mycology (060505) Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800) |
| Divisions: | Current > Research Centres > CRC for Diagnostics Current > QUT Faculties and Divisions > Faculty of Health Past > QUT Faculties & Divisions > Faculty of Science and Technology Current > Institutes > Institute of Health and Biomedical Innovation |
| Copyright Owner: | Copyright 2007 American Society for Microbiology |
| Deposited On: | 16 May 2007 |
| Last Modified: | 29 Feb 2012 20:58 |
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