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Fingerprinting of Campylobacter Jejuni by Using Resolution-Optimised Binary Gene Targets Derived From Comparative Genome Hybridization Studies

Price, Erin, Huygens, Flavia, & Giffard, Philip (2006) Fingerprinting of Campylobacter Jejuni by Using Resolution-Optimised Binary Gene Targets Derived From Comparative Genome Hybridization Studies. Applied and Environmental Microbiology, 72(12), pp. 7793-7803.

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Abstract

A novel method for genotyping the clustered regularly interspaced short palindromic repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods, and emphasizes the potential of HRM for genotyping short sequence repeats in other species.

Impact and interest:

25 citations in Scopus
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21 citations in Web of Science®

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ID Code: 7677
Item Type: Journal Article
Additional Information: For more information, please refer to the journal’s website (see link) or contact the author Flavia Huygens. Author contact details: f.huygens@qut.edu.au
Additional URLs:
Keywords: Campylobacter, Genotyping, Binary Typing, Real-time PCR
DOI: 10.1128/AEM.01338-06
ISSN: 0099-2240
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Mycology (060505)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800)
Divisions: Current > Research Centres > CRC for Diagnostics
Current > QUT Faculties and Divisions > Faculty of Health
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 American Society for Microbiology
Deposited On: 16 May 2007
Last Modified: 29 Feb 2012 20:58

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