Regulatory interplay between pap operons in uropathogenic Escherichia coli

Totsika, Makrina, Beatson, Scott A., Holden, Nicola, & Gally, David L. (2008) Regulatory interplay between pap operons in uropathogenic Escherichia coli. Molecular Microbiology, 67(5), pp. 996-1011.

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Pathogenic bacteria have a large repertoire of surface organelles involved in adherence, motility and protein export, but how individual bacteria co-ordinate surface organelle expression to prevent interference and excessive immune stimulation is unclear. Phase variation is a mechanism by which expression of surface factors is limited to a fraction of the bacterial population; however, the presence of multiple homologous surface structures controlled by related mechanisms and regulators antagonizes the independent expression achieved by phase variation. To investigate whether other mechanisms have evolved to sort out the bacterial cell surface, we examined regulatory cross-talk between multiple phase-variable pyelonephritis-associated pili (pap) operons in Escherichia coli isolates associated with urinary tract infections. Allelic variation identified in the regulatory regions and regulators acts synergistically to limit coexpression of homologous fimbrial operons. In particular, there is evidence that papI is under positive selection and PapI variants displayed differences in their capacity to activate related pap operons. Alleles of the high-affinity binding site for PapB were shown to contain a variable number of (T/A)3 repeats occurring every 9 bp that altered the sensitivity of pap operon activation. Taken together with other examples of surface organelle cross-talk, we illustrate how this regulation could promote sequential expression.

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18 citations in Web of Science®

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ID Code: 77414
Item Type: Journal Article
Refereed: Yes
DOI: 10.1111/j.1365-2958.2007.06098.x
ISSN: 1365-2958
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 The Author(s)
Deposited On: 10 Oct 2014 00:02
Last Modified: 15 Oct 2014 04:00

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