Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073

Holden, Nicola, Totsika, Makrina, Dixon, Lynn, Catherwood, Kirsteen, & Gally, David L. (2007) Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073. Infection and Immunity, 75(7), pp. 3325-3334.

View at publisher (open access)


Adherence of uropathogenic Escherichia coli to host tissue is required for infection and is mediated by fimbriae, such as pyelonephritis-associated pili (Pap). Expression of P fimbriae is regulated by phase variation, and to date, phase transition frequencies have been measured only for pap regulatory region constructs integrated into the E. coli K-12 chromosome. The aim of this work was to measure P phase transition frequencies in clinical isolates for the first time, including frequencies for the sequenced strain E. coli CFT073. P fimbriation and associated phase transition frequencies were measured for two E. coli clinical isolates and compared with levels for homologous pap constructs in E. coli K-12. Fimbriation and off-to-on transition frequencies were always higher in the clinical isolate. It was concluded that the regulatory inputs controlling papI expression are likely to be different in E. coli CFT073 and E. coli K-12 as (i) phase variation could be stimulated in E. coli K-12 by induction of papI and (ii) the level of expression of a papI::gfp+ fusion was higher in E. coli CFT073 than in E. coli K-12. Furthermore, phase transition frequencies for the two E. coli CFT073 pap clusters were shown to be different depending on the culture conditions, indicating that there is a hierarchy of expression depending on signal inputs.

Impact and interest:

12 citations in Scopus
Search Google Scholar™
24 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 77417
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 6 months
DOI: 10.1128/IAI.01989-06
ISSN: 0019-9567
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 American Society for Microbiology
Deposited On: 08 Oct 2014 23:04
Last Modified: 02 Feb 2015 00:16

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page