Pathological excretion patterns of urinary proteins in miners highly exposed to dinitrotoluene

Brüning, T., Thier, R., Mann, H., Melzer, H., Bröde, P., Dallner, G., & Bolt, H. M. (2001) Pathological excretion patterns of urinary proteins in miners highly exposed to dinitrotoluene. Journal of Occupational and Environmental Medicine, 43(7), pp. 610-615.

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Abstract

A cohort of 161 underground miners who had been highly exposed to dinitrotoluene (DNT) in the copper-mining industry of the former German Democratic Republic was reinvestigated for signs of subclinical renal damage. The study included a screening of urinary proteins excreted by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and quantitations of the specific urinary proteins α 1-microglobulin and glutathione-S-transferase α (GST α) as biomarkers for damage of the proximal tubule and glutathione-S-transferase π (GST π) for damage of the distal tubule. The exposures were categorized semiquantitatively (low, medium, high, and very high), according to the type and duration of professional contact with DNT. A straight dose-dependence of pathological protein excretion patterns with the semiquantitative ranking of DNT exposure was seen. Most of the previously reported cancer cases of the urinary tract, especially those in the higher exposed groups, were confined to pathological urinary protein excretion patterns. The damage from DNT was directed toward the tubular system. In many cases, the appearance of Tamm-Horsfall protein, a 105-kD protein marker, was noted. Data on the biomarkers α 1-microglobulin, GST α, and GST π consistently demonstrated a dose-dependent increase in tubular damage, which confirmed the results of screening by SDS-PAGE and clearly indicated a nephrotoxic effect of DNT under the given conditions of exposure. Within the cluster of cancer patients observed among the DNT-exposed workers, only in exceptional cases were normal biomarker excretions found.

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11 citations in Web of Science®

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ID Code: 77450
Item Type: Journal Article
Refereed: Yes
Keywords: 2, 4 dinitrotoluene, 2, 6 dinitrotoluene, alpha 1 microglobulin, copper, glutathione transferase, Tamm Horsfall glycoprotein, toluene derivative, article, human, kidney distal tubule, kidney injury, kidney proximal tubule, major clinical study, mining, nephrotoxicity, occupational exposure, polyacrylamide gel electrophoresis, protein urine level, urinary excretion, urinary tract cancer, Biological Markers, Cohort Studies, Dinitrobenzenes, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Environmental Monitoring, Germany, East, Glutathione S-Transferase pi, Humans, Isoenzymes, Kidney Neoplasms, Membrane Glycoproteins, Proteinuria, Retrospective Studies, Trypsin Inhibitor, Kunitz Soybean
ISSN: 1076-2752
Divisions: Current > Schools > School of Clinical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2001 Lippincott Williams & Wilkins
Deposited On: 17 Oct 2014 00:24
Last Modified: 17 Oct 2014 00:24

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