Rapid analysis of GSTM1, GSTT1 and GSTP1 polymorphisms using real-time polymerase chain reaction

Ko, Y., Koch, B., Harth, V., Sachinidis, A., Thier, R., Vetter, H., Bolt, H. M., & Brüning, T. (2000) Rapid analysis of GSTM1, GSTT1 and GSTP1 polymorphisms using real-time polymerase chain reaction. Pharmacogenetics, 10(3), pp. 271-274.

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Polymorphisms of glutathione transferases (GST) are important genetic determinants of susceptibility to environmental carcinogens (Rebbeck, 1997). The GSTs are a multigene family of dimeric enzymes involved in detoxification, and, in a few cases, the bioactivation of a variety of xenobiotics (Hayes et al., 1995). The cytosolic GST enzyme family consists of four major classes of enzymes, referred to as alpha, mu, pi and theta. Several members of this family (for example, GSTM1, GSTT1 and GSTP1) are polymorphic in human populations (Wormhoudt et al., 1999). Molecular epidemiology studies have examined the role of GST polymorphisms as susceptibility factors for environmentally and/or occupationally induced cancers (Wormhoudt et al., 1999). In particular, case-control studies showed a relationship between the GSTM1 null genotype and the development of cancer in association with smoking habits, which has been shown for cancers of the respiratory and gastrointestinal tracts as well as other cancer types (Miller et al., 1997). Only a few molecular epidemiological studies addressed the role of GSTT1 and GSTP1 polymorphisms in cancer susceptibility. Since GSTP1 is a key player in biotransformation/bioactivation of benzo(a)pyrene, GSTP1 may be even more important than GSTM1 in the prevention of tobacco-induced cancers (Harries et al., 1997; Harris et al., 1998). To date, this relationship has not been sufficiently addressed in humans. Comprehensive molecular epidemiological studies may add to the current knowledge of the role of GST polymorphisms in cancer susceptibility and extent of the knowledge gained from approaches that used phenotyping, such as GSTM1 activity as it relates to trans-stilbene oxide, or polymerase chain reaction (PCR) based genotyping of polymorphic isoenzymes (Bell et al., 1993; Pemble et al., 1994; Harries et al., 1997).

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ID Code: 77466
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: GSTM1, GSTP1, GSTT1, Real-time PCR, glutathione transferase, isoenzyme, xenobiotic agent, article, cancer genetics, controlled study, DNA hybridization, fluorescence, genetic epidemiology, genetic polymorphism, genotype, human, multigene family, pharmacogenetics, polymerase chain reaction, priority journal, DNA Primers, Genetic Screening, Glutathione S-Transferase pi, Humans, Isoenzymes, Polymorphism, Genetic
ISSN: 1744-6880
Divisions: Current > Schools > School of Clinical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2000 Lippincott Williams & Wilkins
Deposited On: 16 Oct 2014 23:59
Last Modified: 16 Oct 2014 23:59

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