A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5 : use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system

Oda, Yoshimitsu, Yamazaki, Hiroshi, Thier, Ricarda, Ketterer, Brian, Guengerich, F. Peter, & Shimada, Tsutomu (1996) A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5 : use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system. Carcinogenesis, 17(2), pp. 297-302.

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The Escherichia coli mu operon was subcloned into a pKK233-2 vector containing rat glutathione S-transferase (GST) 5-5 cDNA and the plasmid thus obtained was introduced into Salmonella typhimurium TA1535. The newly developed strain S.typhimurium NM5004, was found to have 52-fold greater GST activity than the original umu strain S.typhimurium TA1535/pSK1002. We compared sensitivities of these two tester strains, NM5004 and TA1535/ pSK1002, for induction of umuC gene expression with several dihaloalkanes which are activated or inactivated by GST 5-5 activity. The induction of umuC gene expression by these chemicals was monitored by measuring the cellular P-galactosidase activity produced by umuC'lacZ fusion gene in these two tester strains. Ethylene dibromide, 1-bromo-2-chloroethane, 1,2-dichloroethane, and methylene dichloride induced umuC gene expression more strongly in the NM5004 strain than the original strain, 4-Nitroquinoline 1-oxide and N-methyl-N'-nitro-N-nitrosoguanidine were found to induce umuC gene expression to similar extents in both strains. In the case of 1-nitropyrene and 2-nitrofluorene, however, NM5004 strain showed weaker umuC gene expression responses than the original TA1535/ pSK1002 strain, 1,2-Epoxy-3-(4'-nitrophenoxy)propane, a known substrate for GST 5-5, was found to inhibit umuC induction caused by 1-bromo-2-chloroethane. These results indicate that this new tester NM5004 strain expressing a mammalian GST theta class enzyme may be useful for studies of environmental chemicals proposed to be activated or inactivated by GST activity.

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ID Code: 77468
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months
Additional URLs:
Keywords: 1 nitropyrene, 1, 2 dibromoethane, 2 dichloroethane, 2 nitrofluorene, 4 nitroquinoline 1 oxide, alkane, beta galactosidase, chloroethane, dichloromethane, glutathione transferase, halocarbon, methylnitronitrosoguanidine, article, controlled study, enzyme activity, escherichia coli, gene expression, genotoxicity, molecular cloning, nonhuman, operon, plasmid, priority journal, salmonella typhimurium, 4-Nitroquinoline-1-oxide, Animals, Biotransformation, Ethane, Ethylene Dibromide, Ethylene Dichlorides, Fluorenes, Genetic Vectors, Hydrocarbons, Halogenated, Methylene Chloride, Mutagenicity Tests, Pyrenes, Rats, Transfection, Transformation, Bacterial
DOI: 10.1093/carcin/17.2.297
ISSN: 1460-2180
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Schools > School of Early Childhood & Inclusive Education
Copyright Owner: Copyright 1996 Oxford University Press
Deposited On: 17 Oct 2014 01:08
Last Modified: 28 Jan 2015 02:25

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