Determination of glutathione transferase (GSTT1-1) activities in different tissues based on formation of radioactive metabolites using 35S-glutathione

Thier, Ricarda, Delbanco, Evert H., Wiebel, Frederike A., Hallier, Ernst, & Bolt, Hermann M. (1998) Determination of glutathione transferase (GSTT1-1) activities in different tissues based on formation of radioactive metabolites using 35S-glutathione. Archives of Toxicology, 72(12), pp. 811-815.

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Abstract

A new system has been developed to determine enzyme activities of glutathione transferase θ (GSTT1-1) based on radiometric product detection resulting from the enzymic reaction of methyl chloride with 35S-labelled glutathione. In principle, the method is universally applicable for determination of glutathione transferase activities towards a multiplicity of substrates. The method distinguishes between erythrocyte GSTT1-1 activities of human 'non-conjugators', 'low conjugators' and 'high conjugators'. Application to cytosol preparations of livers and kidneys of male and female Fischer 344 and B6C3F1 mice reveals differential GSTT1-1 activities in hepatic and renal tissues. These ought to be considered in species-specific modellings of organ toxicities of chlorinated hydrocarbons.

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8 citations in Web of Science®

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ID Code: 77483
Item Type: Journal Article
Refereed: Yes
Keywords: Glutathione transferase GSTT1, Methyl chloride, Radiometric assay, Tissue specificity, chlorinated hydrocarbon, glutathione, glutathione transferase, sulfur 35, animal tissue, article, controlled study, enzyme activity, enzyme assay, enzyme substrate, female, kidney parenchyma, liver cytosol, male, mouse, nonhuman, priority journal, radiometry, rat, species difference, tissue level, xenobiotic metabolism, Animals, Erythrocytes, Humans, Kidney, Liver, Mice, Organ Specificity, Rats, Rats, Inbred F344, Substrate Specificity, Sulfur Radioisotopes
DOI: 10.1007/s002040050578
ISSN: 0340-5761
Divisions: Current > Schools > School of Clinical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 1998 Springer-Verlag
Deposited On: 15 Oct 2014 23:19
Last Modified: 15 Oct 2014 23:19

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