Biological monitoring in workers in a nitrobenzene reduction plant : Haemoglobin versus serum albumin adducts

Thier, R., Lewalter, J., Selinski, S., & Bolt, H. M. (2001) Biological monitoring in workers in a nitrobenzene reduction plant : Haemoglobin versus serum albumin adducts. International Archives of Occupational and Environmental Health, 74(7), pp. 483-488.

View at publisher


The high priority of monitoring workers exposed to nitrobenzene is a consequence of clear findings of experimental carcinogenicity of nitrobenzene and the associated evaluations by the International Agency for Research on Cancer. Eighty male employees of a nitrobenzene reduction plant, with potential skin contact with nitrobenzene and aniline, participated in a current medical surveillance programme. Blood samples were routinely taken and analysed for aniline, 4-aminodiphenyl (4-ADP) and benzidine adducts of haemoglobin (Hb) and human serum albumin (HSA). Also, levels of methaemoglobin (Met-Hb) and of carbon monoxide haemoglobin (CO-Hb) were monitored. Effects of smoking were straightforward. Using the rank sum test of Wilcoxon, we found that very clear-cut and statistically significant smoking effects (about 3-fold increases) were apparent on CO-Hb (P = 0.00085) and on the Hb adduct of 4-ADP (P = 0.0006). The mean aniline-Hb adduct level in smokers was 1.5 times higher than in non-smokers; the significance (P = 0.05375) was close to the 5% level. The strongest correlation was evident between the Hb and HSA adducts of aniline (rs = 0.846). Less pronounced correlations (but with P values < 0.02) appeared between aniline-Hb and 4-ADP-Hb adducts (rs = 0.388), between 4-ADP and 4-ADP-HSA adducts (rs = 0.373), and between 4-ADP-Hb and aniline-HSA adducts (rs = 0.275). In view of the proposal for additional use of the aniline-HSA adduct for biological monitoring, particularly in cases of acute overexposures or poisonings, the strong correlation of the Hb and HSA conjugates is noteworthy; the ratio aniline-HSA:aniline-Hb was 1:42 for the entire cohort.

Impact and interest:

8 citations in Scopus
8 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 77488
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Aniline, Biological monitoring, Haemoglobin adducts, Nitrobenzene, Serum albumin adducts, 4 biphenylamine, benzidine, carboxyhemoglobin, hemoglobin, human serum albumin, methemoglobin, article, blood sampling, cancer risk, carcinogenicity, cohort analysis, controlled study, correlation function, employee, health survey, human, industry, major clinical study, male, occupational exposure, protein interaction, skin penetration, smoking, statistical analysis, worker, Chemical Industry, Environmental Monitoring, Hemoglobins, Humans, Nitrobenzenes, Occupational Health, Serum Albumin
DOI: 10.1007/s004200100250
ISSN: 1432-1246
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2001 Springer-Verlag
Deposited On: 16 Oct 2014 00:16
Last Modified: 22 Jun 2017 23:01

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page