Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells

Coso, Sanja, Zeng, Yiping, Sooraj, Dhanya, & Williams, Elizabeth D. (2011) Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells. Experimental Cell Research, 317(17), pp. 2397-2407.

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Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function.

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3 citations in Scopus
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3 citations in Web of Science®

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ID Code: 77743
Item Type: Journal Article
Refereed: Yes
Keywords: Lymphatic endothelial cell, Migration, Vascular endothelial growth factor
DOI: 10.1016/j.yexcr.2011.07.023
ISSN: 0014-4827
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 16 Oct 2014 23:31
Last Modified: 20 Oct 2014 01:43

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