Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens
Punyadeera, C., Dunselman, G., Marbaix, E., Kamps, R., Galant, C., Nap, A., de Goeij, A., Ederveen, A., & Groothuis, P. (2004) Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens. Journal of Steroid Biochemistry and Molecular Biology, 92(3), pp. 175-185.
The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human proliferative phase endometrium using short-term explant cultures. The effects of oestrogen (17beta-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished:
(1) the menstrual (M) phase pattern (cycle days 2-5), which is characterised by a complete lack in the proliferative response to 17beta-E2, while an increased expression of AR (2.6-fold, P<0.01), PR (2.7-fold, P<0.01) and COX-2 (3.5-fold, P<0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level;
(2) the early proliferative (EP) phase pattern (cycle days 6-10) with 17beta-E2 enhanced proliferation in the stroma (1.7-fold, P<0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-a mRNA and protein levels were significantly reduced by 17beta-E2;
(3) the late proliferative (LP) phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P<0.05) and PR mRNA expression (1.7-fold, P<0.01) by 17beta-E2. In conclusion, three distinct response patterns to 17beta-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human proliferative phase endometrium explant cultures.
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|Item Type:||Journal Article|
|Additional Information:||<Go to ISI>://WOS:000226009000007|
|Keywords:||steroid receptors, steroid hormones, maturation, explant cultures, normal menstrual-cycle, prostaglandin-f2-alpha output, matrix, metalloproteinases, receptor content, messenger-rna, expression, progesterone, tissue, cells, estradiol|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
|Deposited On:||23 Oct 2014 00:57|
|Last Modified:||24 Oct 2014 01:08|
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