Three-dimensional flow-through protein platform

van Lieshout, R. M. L., van Domburg, T., Saalmink, M., Verbeek, R., Wimberger-Friedl, R., van Dieijen-Visser, M. P., & Punyadeera, Chamindie (2009) Three-dimensional flow-through protein platform. Analytical Chemistry, 81(13), pp. 5165-5171.

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Abstract

We have developed a new protein microarray (Immuno-Flow Protein Platform, IFPP) that utilizes a porous nitrocellulose (NC) membrane with printed spots of capture probes. The sample is pumped actively through the NC membrane, to enhance binding efficiency and introduce stringency. Compared to protein microarrays assayed with the conventional incubation-shaking method the rate of binding is enhanced on the IFPP by at least a factor of 10, so that the total assay time can be reduced drastically without compromising sensitivity. Similarly, the sensitivity can be improved. We demonstrate the detection of 1 pM of C-reactive protein (CRP) in 70 mu L of plasma within a total assay time of 7 min. The small sample and reagent volumes, combined with the speed of the assay, make our IFPP also well-suited for a point-of-care/near-patient setting. The potential clinical application of the IFPP is demonstrated by validating CRP detection both in human plasma and serum samples against standard clinical laboratory methods.

Impact and interest:

11 citations in Scopus
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10 citations in Web of Science®

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ID Code: 77878
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: c-reactive protein, microarrays, sensitivity, assay, technology, hydrogel, kinetics, chips
DOI: 10.1021/ac801244d
ISSN: 0003-2700
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright © 2009 American Chemical Society
Deposited On: 22 Oct 2014 04:09
Last Modified: 23 Oct 2014 00:55

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