One-step homogeneous C-reactive protein assay for saliva

Punyadeera, Chamindie, Dimeski, Goce, Kostner, Karam, Beyerlein, Peter, & Cooper-White, Justin (2011) One-step homogeneous C-reactive protein assay for saliva. Journal of Immunological Methods, 373(1-2), pp. 19-25.

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Abstract

Background: Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting. Methods: We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15 min (vs 90 min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n = 55, ages 20-70 years) as well as from cardiac patients (n = 28, ages 43-86 years).

Results: The assay incubation time was optimised from 90 min to 15 mm and generated a positive correlation (n = 29, range 10-2189 pg/mL, r2 = 0.94; Passing Bablok slope 0.885. Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285 pg/mL and in cardiac patients was 1680 pg/mL (p<0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2 = 0.84, p<0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients. Conclusions: The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events. (C) 2011 Elsevier B.V. All rights reserved.

Impact and interest:

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ID Code: 77925
Item Type: Journal Article
Refereed: Yes
Additional Information: Times Cited: 24
Punyadeera, Chamindie Dimeski, Goce Kostner, Karam Beyerlein, Peter Cooper-White, Justin
Queensland Government; University of Queensland [601252]
The authors would like to acknowledge the financial support of the Queensland Government Smart Futures Fellowship Programme (QGSFF), and the University of Queensland New Staff Research Funds (UQNSRSF 601252). The authors wish to acknowledge Professor Maree Smith and her team at TetraQ for the access to the Envison plate reader.
Keywords: Human saliva, High-sensitive C-reactive protein assay, Homogeneous bead, based assay, Cardiovascular risk factors, Non-invasive, cardiovascular-disease, quantification, diagnosis
DOI: 10.1016/j.jim.2011.07.013
ISSN: 0022-1759
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2011 Elsevier BV
Deposited On: 22 Oct 2014 00:32
Last Modified: 23 Oct 2014 03:20

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