Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry
Bailey, Ulla-Maja, Punyadeera, Chamindie, Cooper-White, Justin J., & Schulz, Benjamin L. (2012) Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 911, pp. 21-26.
Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic isoforms. Our results emphasize the importance of consideration of post-translational events such as proteolysis in proteomic studies, biomarker discovery and validation, particularly in saliva. (C) 2012 Elsevier B.V. All rights reserved.
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|Item Type:||Journal Article|
|Additional Information:||Times Cited: 6 Bailey, Ulla-Maja Punyadeera, Chamindie Cooper-White, Justin J. Schulz, Benjamin L. NHMRC ; Career Development Fellowship [APP1031542]; Queensland Government Smart Futures Fellowship; University of Queensland Foundation Research Excellence Award Financial support for this work was provided by NHMRC project grant 631615 and Career Development Fellowship APP1031542 to BLS, and Queensland Government Smart Futures Fellowship and The University of Queensland Foundation Research Excellence Award to CP.|
|Keywords:||Saliva, Alpha-amylase, Liquid chromatography, Mass spectrometry, Proteolysis, Biomarker, proteins, identification, profiles, binding, tannin, forms|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2012 Elsevier BV|
|Deposited On:||22 Oct 2014 00:48|
|Last Modified:||23 Oct 2014 03:26|
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