NT-ProBNP levels in saliva and its clinical relevance to heart failure

Foo, Jared Yong Yang, Wan, Yunxia, Kostner, Karam, Arivalagan, Alicia, Atherton, John, Cooper-White, Justin, Dimeski, Goce, & Punyadeera, Chamindie (2012) NT-ProBNP levels in saliva and its clinical relevance to heart failure. Plos One, 7(10), e48452.

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Background: Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body's health and well being and similar to 20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non-invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels.

Methods: Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at -80 degrees C until analysis. A customised homogeneous sandwich AlphaLISA((R)) immunoassay was used to quantify NT-proBNP levels in saliva.

Results: Our NT-proBNP immunoassay was validated against a commercial Roche assay on plasma samples collected from HF patients (n = 37) and the correlation was r(2) = 0.78 (p<0.01, y = 1.705 x +1910.8). The median salivary NT-proBNP levels in the healthy and HF participants were <16 pg/mL and 76.8 pg/mL, respectively. The salivary NT-proBNP immunoassay showed a clinical sensitivity of 82.2% and specificity of 100%, positive predictive value of 100% and negative predictive value of 83.3%, with an overall diagnostic accuracy of 90.6%.

Conclusion: We have firstly demonstrated that NT-proBNP can be detected in saliva and that the levels were higher in heart failure patients compared with healthy control subjects. Further studies will be needed to demonstrate the clinical relevance of salivary NT-proBNP in unselected, previously undiagnosed populations.

Impact and interest:

17 citations in Scopus
11 citations in Web of Science®
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ID Code: 77927
Item Type: Journal Article
Refereed: Yes
Additional Information: Times Cited: 4
Foo, Jared Yong Yang Wan, Yunxia Kostner, Karam Arivalagan, Alicia Atherton, John Cooper-White, Justin Dimeski, Goce Punyadeera, Chamindie
Queensland Government Smart Futures Fellowship Programme (QGSFF); University of Queensland New Staff Research Funds (UQNSRSF) [601252]; University of Queensland Foundation Research Excellence Award Scheme
The authors would like to acknowledge the financial support of the Queensland Government Smart Futures Fellowship Programme (QGSFF), the University of Queensland New Staff Research Funds (UQNSRSF 601252) and the University of Queensland Foundation Research Excellence Award Scheme. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords: brain natriuretic peptide, diagnostic-accuracy, task-force, association, guidelines, management, bnp, immunoassays, convertases, people
DOI: 10.1371/journal.pone.0048452
ISSN: 1932-6203
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Nursing
Copyright Owner: Copyright 2012 Foo et al.
Copyright Statement: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Deposited On: 22 Oct 2014 02:03
Last Modified: 23 Oct 2014 03:34

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