Evaluation of saliva collection devices for the analysis of proteins

Topkas, Eleni, Keith, Patricia, Dimeski, Goce, Cooper-White, Justin, & Punyadeera, Chamindie (2012) Evaluation of saliva collection devices for the analysis of proteins. Clinica Chimica Acta, 413(13-14), pp. 1066-1070.

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Abstract

Background: Human saliva mirrors the body's health and can be collected non-invasively, does not require specialized skills and is suitable for large population based screening programs. The aims were twofold: to evaluate the suitability of commercially available saliva collection devices for quantifying proteins present in saliva and to provide levels for C-reactive protein (CRP), myoglobin, and immunoglobin E (IgE) in saliva of healthy individuals as a baseline for future studies.

Methods: Saliva was collected from healthy volunteers (n = 17, ages 18-33 years). The following collection methods were evaluated: drool; Salimetrics (R) Oral Swab (SOS); Salivette (R) Cotton and Synthetic (Sarstedt) and Greiner Bio-One Saliva Collection System (GBO SCS (R)). We used AlphaLISA (R) assays to measure CRP, IgE and myoglobin levels in human saliva.

Results: Significant (p<0.05) differences in the salivary flow rates were observed based on the method of collection, Le. salivary flow rates were significantly lower (p<0.05) in unstimulated saliva (Le. drool and SOS), when compared with mechanically stimulated methods (p<0.05) (Salivette (R) Cotton and Synthetic) and acid stimulated method (p<0.05) (SCS (R)). Saliva collected using SOS yielded significantly (p<0.05) lower concentrations of myoglobin and CRP, whilst, saliva collected using the Salivette (R) Cotton and Synthetic swab yielded significantly (p<0.05) lower myoglobin and IgE concentrations respectively.

Conclusions: The results demonstrated significantly relevant differences in analyte levels based on the collection method. Significant differences in the salivary flow rates were also observed depending on the saliva collection method. The data provide preliminary baseline values for salivary CRP, myoglobin, and IgE levels in healthy participants and based on the collection method. (C) 2012 Elsevier B.V. All rights reserved.

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ID Code: 77931
Item Type: Journal Article
Refereed: Yes
Additional Information: Times Cited: 25
Topkas, Eleni Keith, Patricia Dimeski, Goce Cooper-White, Justin Punyadeera, Chamindie
Queensland Government Smart Futures Fellowship Programme (QGSFF); University of Queensland New Staff Research Funds (UQNSRSF) [601252]; University of Queensland Foundation
The authors would like to acknowledge the financial support from the Queensland Government Smart Futures Fellowship Programme (QGSFF), the University of Queensland New Staff Research Funds (UQNSRSF 601252) and The University of Queensland Foundation Research Excellence Award. The authors wish to acknowledge Ms. Thea Cullen for her assistance in data collection. In addition, authors wish to acknowledge PerkinElmer for the supply of Beta-Test kits.
Keywords: Saliva, Diagnostic, Proteins, Saliva collection devices and methods, Reference intervals, c-reactive protein, health, assay, diagnostics, steroids, disease, drugs, fluid, care
DOI: 10.1016/j.cca.2012.02.020
ISSN: 0009-8981
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2012 Elsevier B.V.
Deposited On: 22 Oct 2014 04:27
Last Modified: 23 Oct 2014 04:12

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