Quantification of D-dimer levels in human saliva

Zhang, X., Wan, Y. X., Cooper-White, J., Dimeski, G., Atherton, J., & Punyadeera, Chamindie (2013) Quantification of D-dimer levels in human saliva. Bioanalysis, 5(18), pp. 2249-2256.

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Abstract

Background: Plasma D-dimer tests are currently used to exclude deep vein thrombosis and pulmonary embolism. Human saliva has numerous advantages over blood as a diagnostic sample. The aims of our study were to develop a reliable immunoassay to detect D-dimer levels in saliva, and to determine the correlation between salivary and blood D-dimer levels. Results/methodology: Saliva and blood samples were collected from 40 healthy volunteers. We developed a AlphaLISA((R)) immunoassay with acceptable analytical performances to quantify D-dimer levels in the samples. The median salivary D-dimer levels were 138.1 ng/ml (morning) and 140.7 ng/ml (afternoon), and the plasma levels were 75.0 ng/ml. Salivary D-dimer levels did not correlate with plasma levels (p = 0.61). Conclusion: For the first time, we have quantified D-dimer levels and found twofold increase in saliva (p < 0.05) than in plasma. Further studies are required to demonstrate the clinical relevance/utility of salivary D-dimer in patients with confirmed deep vein thrombosis and/or pulmonary embolism.

Impact and interest:

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3 citations in Web of Science®

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ID Code: 77961
Item Type: Journal Article
Refereed: Yes
Keywords: deep-vein thrombosis, c-reactive protein, fibrin d-dimer, pulmonary-embolism, venous thromboembolism, cancer, diagnosis, head
DOI: 10.4155/bio.13.190
ISSN: 1757-6180
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2013 Future Science Ltd.
Deposited On: 23 Oct 2014 01:40
Last Modified: 24 Oct 2014 02:00

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