Quantification of D-dimer levels in human saliva

Zhang, X., Wan, Y. X., Cooper-White, J., Dimeski, G., Atherton, J., & Punyadeera, Chamindie (2013) Quantification of D-dimer levels in human saliva. Bioanalysis, 5(18), pp. 2249-2256.

View at publisher


Background: Plasma D-dimer tests are currently used to exclude deep vein thrombosis and pulmonary embolism. Human saliva has numerous advantages over blood as a diagnostic sample. The aims of our study were to develop a reliable immunoassay to detect D-dimer levels in saliva, and to determine the correlation between salivary and blood D-dimer levels. Results/methodology: Saliva and blood samples were collected from 40 healthy volunteers. We developed a AlphaLISA((R)) immunoassay with acceptable analytical performances to quantify D-dimer levels in the samples. The median salivary D-dimer levels were 138.1 ng/ml (morning) and 140.7 ng/ml (afternoon), and the plasma levels were 75.0 ng/ml. Salivary D-dimer levels did not correlate with plasma levels (p = 0.61). Conclusion: For the first time, we have quantified D-dimer levels and found twofold increase in saliva (p < 0.05) than in plasma. Further studies are required to demonstrate the clinical relevance/utility of salivary D-dimer in patients with confirmed deep vein thrombosis and/or pulmonary embolism.

Impact and interest:

6 citations in Scopus
5 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 77961
Item Type: Journal Article
Refereed: Yes
Keywords: deep-vein thrombosis, c-reactive protein, fibrin d-dimer, pulmonary-embolism, venous thromboembolism, cancer, diagnosis, head
DOI: 10.4155/bio.13.190
ISSN: 1757-6180
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2013 Future Science Ltd.
Deposited On: 23 Oct 2014 01:40
Last Modified: 23 Jun 2017 02:02

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page