A staining protocol for identifying secondary compounds in Myrtaceae

Retamales, Hernan A. & Scharaschkin, Tanya (2014) A staining protocol for identifying secondary compounds in Myrtaceae. Applications in Plant Sciences, 2(10).

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• Premise of the study: Here we propose a staining protocol using TBO and Ruthenium red in order to reliably identify secondary compounds in the leaves of some species of Myrtaceae.

• Methods and results: Leaves of 10 species representing 10 different genera of Myrtaceae were processed and stained using five different combinations of Ruthenium red and TBO. Optimal staining conditions were determined as 1 min of Ruthenium red (0.05% aqueous) and 45 sec of TBO (0.1% aqueous). Secondary compounds clearly identified under this treatment include mucilage in mesophyll, polyphenols in cuticle, lignin in fibers and xylem, tannins and carboxylated polysaccharides in epidermis and pectic substances in primary cell walls.

• Conclusions: Potential applications of this protocol include systematic, phytochemical and ecological investigations in Myrtaceae. It might be applicable to other plant families rich in secondary compounds and could be used as preliminary screening method for extraction of these elements.

Impact and interest:

1 citations in Scopus
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ID Code: 78059
Item Type: Journal Article
Refereed: Yes
Keywords: anatomy, Myrtaceae, ruthenium red, staining, TBO
DOI: 10.3732/apps.1400063
ISSN: 2168-0450
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Biology not elsewhere classified (060799)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
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Copyright Owner: Copyright 2014 Retamales and Scharaschkin. Published by the Botanical Society of America.
Copyright Statement: This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
Deposited On: 28 Oct 2014 00:38
Last Modified: 30 Oct 2014 04:50

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