Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket
Kalaivani, G., Narayanan, N.S.V., Arumugam , Sivanesan, Kannan, A., Kaminska, A., & Sevvel, R. (2013) Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket. RSC Advances, 3(19), pp. 6839-6845.
Shell isolated silver nanoparticles with an ultrathin silica layer (Ag@SiO2NPs) are used as a surface-enhanced resonance Raman scattering (SERRS) substrate for probing metmyoglobin (metMb) in aqueous solution. The ultrathin silica layer protects metMb from reaching the bare silver surface and conserves the heme pocket during SERRS analysis with a Raman enhancement factor (EFSERS) of 4.78 × 104. In spite of the good SERRS enhancement, the interaction between the protein and Ag@SiO2NPs is weak enough to separate them by centrifugation in such a way that both are regenerated in their original form and can be reused. Using Ag@SiO2NPs as the SERRS substrate, the lowest detection limit of 2 nM was achieved for metMb whilst conserving the native structure of the heme centre.
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|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2013 R S C Publications|
|Deposited On:||10 Nov 2014 02:04|
|Last Modified:||11 Nov 2014 04:39|
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