Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket

Kalaivani, G., Narayanan, N.S.V., Arumugam , Sivanesan, Kannan, A., Kaminska, A., & Sevvel, R. (2013) Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket. RSC Advances, 3(19), pp. 6839-6845.

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Shell isolated silver nanoparticles with an ultrathin silica layer (Ag@SiO2NPs) are used as a surface-enhanced resonance Raman scattering (SERRS) substrate for probing metmyoglobin (metMb) in aqueous solution. The ultrathin silica layer protects metMb from reaching the bare silver surface and conserves the heme pocket during SERRS analysis with a Raman enhancement factor (EFSERS) of 4.78 × 104. In spite of the good SERRS enhancement, the interaction between the protein and Ag@SiO2NPs is weak enough to separate them by centrifugation in such a way that both are regenerated in their original form and can be reused. Using Ag@SiO2NPs as the SERRS substrate, the lowest detection limit of 2 nM was achieved for metMb whilst conserving the native structure of the heme centre.

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5 citations in Scopus
5 citations in Web of Science®
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ID Code: 78415
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1039/C3RA40842F
ISSN: 2046-2069
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 R S C Publications
Deposited On: 10 Nov 2014 02:04
Last Modified: 23 Jun 2017 05:01

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