Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket

Kalaivani, G., Narayanan, N.S.V., Arumugam , Sivanesan, Kannan, A., Kaminska, A., & Sevvel, R. (2013) Regenerative silver nanoparticles for SERRS investigation of metmyoglobin with conserved heme pocket. RSC Advances, 3(19), pp. 6839-6845.

View at publisher

Abstract

Shell isolated silver nanoparticles with an ultrathin silica layer (Ag@SiO2NPs) are used as a surface-enhanced resonance Raman scattering (SERRS) substrate for probing metmyoglobin (metMb) in aqueous solution. The ultrathin silica layer protects metMb from reaching the bare silver surface and conserves the heme pocket during SERRS analysis with a Raman enhancement factor (EFSERS) of 4.78 × 104. In spite of the good SERRS enhancement, the interaction between the protein and Ag@SiO2NPs is weak enough to separate them by centrifugation in such a way that both are regenerated in their original form and can be reused. Using Ag@SiO2NPs as the SERRS substrate, the lowest detection limit of 2 nM was achieved for metMb whilst conserving the native structure of the heme centre.

Impact and interest:

4 citations in Scopus
Search Google Scholar™
5 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 78415
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1039/C3RA40842F
ISSN: 2046-2069
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 R S C Publications
Deposited On: 10 Nov 2014 02:04
Last Modified: 11 Nov 2014 04:39

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page