Plasmon-tuned silver colloids for SERRS analysis of methemoglobin with preserved nativity

Kalaivani, G., Arumugam , Sivanesan, Kannan, A., Venkata Narayanan, N. S., Kaminska, A., & Sevvel, R. (2012) Plasmon-tuned silver colloids for SERRS analysis of methemoglobin with preserved nativity. Langmuir, 28(40), pp. 14357-14363.

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Optically tuned silver nanoparticles (AgNP's) functionalized with ω-mercaptoalkanoic acids are synthesized and used as a signal amplifier for the surface-enhanced resonance Raman scattering (SERRS) study of heme cofactor in methemoglobin (metHb). Even though both mercaptopropionic acid (MPA)- and mercaptononanoic acid (MNA)-functionalized AgNP's exemplify vastly enhanced SERRS signal of metHb, MNA-AgNP's amplify the SERRS signal amid preservation of the nativity of the heme pocket, unlike MPA-AgNP's. The electrostatic interaction between MNA-AgNP's and metHb leads to instant signal enhancement with a Raman enhancement factor (EF(SERS)) of 4.2 × 10(3). Additionally, a Langmuir adsorption isotherm has been employed for the adsorption of metHb on the MNA-AgNP surface, which provides the real surface coverage and equilibrium constant (K) of metHb as 139 nM and 3.6 × 10(8) M(-1), respectively. The lowest detection limit of 10 nM for metHb has been demonstrated using MNA-AgNP's besides retaining the nativity of the heme pocket.

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ID Code: 78417
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1021/la303136v
ISSN: 1520-5827
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2012 American Chemical Society
Deposited On: 10 Nov 2014 01:45
Last Modified: 11 Nov 2014 05:08

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