Electrochemical current rectification – a novel signal amplification strategy for highly sensitive and selective aptamer-based biosensor

Feng, Lingyan, Sivanesan, Arumugam, Lyu, Zhaozi, Offenhäusser, Andreas, & Mayer, Dirk (2015) Electrochemical current rectification – a novel signal amplification strategy for highly sensitive and selective aptamer-based biosensor. Biosensors and Bioelectronics, 66, pp. 62-68.

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Electrochemical aptamer-based (E-AB) sensors represent an emerging class of recently developed sensors. However, numerous of these sensors are limited by a low surface density of electrode-bound redox-oligonucleotides which are used as probe. Here we propose to use the concept of electrochemical current rectification (ECR) for the enhancement of the redox signal of E-AB sensors. Commonly, the probe-DNA performs a change in conformation during target binding and enables a nonrecurring charge transfer between redox-tag and electrode. In our system, the redox-tag of the probe-DNA is continuously replenished by solution-phase redox molecules. A unidirectional electron transfer from electrode via surface-linked redox-tag to the solution-phase redox molecules arises that efficiently amplifies the current response. Using this robust and straight-forward strategy, the developed sensor showed a substantial signal amplification and consequently improved sensitivity with a calculated detection limit of 114 nM for ATP, which was improved by one order of magnitude compared with the amplification-free detection and superior to other previous detection results using enzymes or nanomaterials-based signal amplification. To the best of our knowledge, this is the first demonstration of an aptamer-based electrochemical biosensor involving electrochemical rectification, which can be presumably transferred to other biomedical sensor systems.

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ID Code: 78574
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/j.bios.2014.10.057
ISSN: 0956-5663
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2014 Elsevier
Copyright Statement: This is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors and Bioelectronics, [VOL 66 (2015)] DOI: 10.1016/j.bios.2014.10.057
Deposited On: 10 Nov 2014 22:59
Last Modified: 13 Nov 2015 08:04

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