Detection of DNA mutations using novel SERS (Surface-Enhanced Raman Spectroscopy) diagnostic platform

Kamińska, Agnieszka , Sivanesan, Arumugam, Witkowska, Evelin, Gołąb, Jakub, Winiarska, Magdalena, Nowis, Dominika, Dzięcielewski, Igor, Weyher, Jan L., & Waluk, Jacek (2013) Detection of DNA mutations using novel SERS (Surface-Enhanced Raman Spectroscopy) diagnostic platform. Journal of Chemistry and Chemical Engineering, 7(10), pp. 972-978.

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Abstract

This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS (surface-enhanced Raman spectroscopy) diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL (breakpoint cluster region-Abelson) gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA (single-strand DNA) was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.

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ID Code: 78575
Item Type: Journal Article
Refereed: Yes
ISSN: 1934-7383
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 [please consult the author]
Deposited On: 10 Nov 2014 23:08
Last Modified: 12 Nov 2014 00:19

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