Evaluation of the efficacy of radiation-modifying compounds using γH2AX as a molecular marker of DNA double-strand breaks

Mah, Li-Jeen, Orlowski, Christian, Ververis, Katherine, Vasireddy, Raja S., El-Osta, Assam, & Karagiannis, Tom C. (2011) Evaluation of the efficacy of radiation-modifying compounds using γH2AX as a molecular marker of DNA double-strand breaks. Genome Integrity, 2(3).

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Abstract

Radiation therapy is a widely used therapeutic approach for cancer. To improve the efficacy of radiotherapy there is an intense interest in combining this modality with two broad classes of compounds, radiosensitizers and radioprotectors. These either enhance tumour-killing efficacy or mitigate damage to surrounding non-malignant tissue, respectively. Radiation exposure often results in the formation of DNA double-strand breaks, which are marked by the induction of H2AX phosphorylation to generate γH2AX. In addition to its essential role in DDR signalling and coordination of double-strand break repair, the ability to visualize and quantitate γH2AX foci using immunofluorescence microscopy techniques enables it to be exploited as an indicator of therapeutic efficacy in a range of cell types and tissues. This review will explore the emerging applicability of γH2AX as a marker for monitoring the effectiveness of radiation-modifying compounds.

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16 citations in Scopus
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ID Code: 78899
Item Type: Journal Article
Refereed: Yes
Keywords: radiation therapy, DNA double-strand breaks, immunofluorescence microscopy, radiation-modifying compounds
DOI: 10.1186/2041-9414-2-3
ISSN: 2041-9414
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Copyright Owner: Copyright 2011 Mah et al. Licensee BioMed Central Ltd.
Copyright Statement: This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
Deposited On: 24 Nov 2014 23:15
Last Modified: 26 Nov 2014 03:32

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