Development of a transient, high-level expression platform for protein production in plants

Norkunas, Karlah-Jade (2014) Development of a transient, high-level expression platform for protein production in plants. PhD thesis, Queensland University of Technology.


Plants are an attractive alternative to conventional expression systems for the production of recombinant proteins and useful biologics, however, the economic viability of plant made proteins is strongly yield dependent. This study aimed to improve transgene expression levels in the plant host Nicotiana benthamiana using the Agroinfiltration transient expression platform. Independent investigation of the physical, chemical and genetic features associated with Agroinfiltration identified factors that improved transformation frequencies, elevated transgene expression levels and ultimately improved protein yield. The major outcome of this research was a novel hyper-expression system for biofarming recombinant proteins in plants.

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ID Code: 78980
Item Type: QUT Thesis (PhD)
Supervisor: Dugdale, Benjamin, Harding, Rob, & Dale, James
Keywords: Agrobacterium-mediated transformation , Biofarming, Optimal Transgenesis (OPTrans), Hyper-expression, Nicotiana benthamiana, Recombinant proteins, pEAQ-HT
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Institution: Queensland University of Technology
Deposited On: 17 Dec 2014 04:13
Last Modified: 08 Sep 2015 06:51

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