Altering the redox state of skeletal muscle by glutathione depletion increases the exercise-activation of PGC-1α
Strobel, Natalie, Matsumoto, Aya, Peake, Jonathan, Marsh, Susan, Peternelj, Tina, Briskey, David, Fassett, Robert, & Coombes, Jeff S. (2014) Altering the redox state of skeletal muscle by glutathione depletion increases the exercise-activation of PGC-1α. Physiological Reports, 2(12), e12224.
We investigated the relationship between mitochondrial biogenesis, cell signalling and antioxidant enzymes by depleting skeletal muscle glutathione with diethyl maleate (DEM) which resulted in a demonstrable increase in oxidative stress during exercise. Animals were divided into six groups:
(1) sedentary control rats;
(2) sedentary rats treated with DEM;
(3) exercise control rats euthanized immediately after exercise;
(4) exercise rats + DEM;
(5) exercise control rats euthanized 4 h after exercise, and;
(6) exercise rats + DEM euthanized 4 h after exercise.
Exercising animals ran on the treadmill at a 10% gradient at 20 m/min for the first 30 min. The speed was then increased every 10 min by 1.6 m/min until exhaustion. There was a reduction in total glutathione in the skeletal muscle of DEM treated animals compared to the control animals (P<0.05). Within the control group, total glutathione was higher in the sedentary group compared to after exercise (P<0.05). DEM treatment also significantly increased oxidative stress, as measured by increased plasma F2-isoprostanes (P<0.05). Exercising animals given DEM showed a significantly greater increase in peroxisome proliferator activated receptor γ coactivator-1α(PGC-1α) mRNA compared to the control animals that were exercised (P<0.05). This study provides novel evidence that by reducing the endogenous antioxidant glutathione in skeletal muscle and inducing oxidative stress through exercise, PGC-1α gene expression was augmented. These findings further highlight the important role of exercise induced oxidative stress in the regulation of mitochondrial biogenesis.
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|Item Type:||Journal Article|
|Additional Information:||Articles free to read on journal website|
|Keywords:||reactive oxygen species; diethy l maleate; exercise; PGC-1 α|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > HUMAN MOVEMENT AND SPORTS SCIENCE (110600) > Exercise Physiology (110602)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2015 [The Authors]|
|Copyright Statement:||All articles accepted from 14 August 2012 are published under the terms of the Creative Commons Attribution License. All articles accepted before this date, were published under a Creative Commons Attribution Non-Commercial License.|
|Deposited On:||16 Dec 2014 00:22|
|Last Modified:||29 Jan 2015 03:56|
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