Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry

Maccarone, Alan T., Duldig, Jackson, Mitchell, Todd W., Blanksby, Stephen J., Duchoslav, Eva, & Campbell, J. Larry (2014) Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry. Journal of Lipid Research, 55(8), pp. 1668-1677.

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Abstract

Glycerophospholipids (GPs) that differ in the relative position of the two fatty acyl chains on the glycerol backbone (i.e., sn-positional isomers) can have distinct physicochemical properties. The unambiguous assignment of acyl chain position to an individual GP represents a significant analytical challenge. Here we describe a workflow where phosphatidylcholines (PCs) are subjected to ESI for characterization by a combination of differential mobility spectrometry and MS (DMS-MS). When infused as a mixture, ions formed from silver adduction of each phospholipid isomer {e.g., [PC (16:0/18:1) + Ag]+ and [PC (18:1/16:0) + Ag]+} are transmitted through the DMS device at discrete compensation voltages. Varying their relative amounts allows facile and unambiguous assignment of the sn-positions of the fatty acyl chains for each isomer. Integration of the well-resolved ion populations provides a rapid method (< 3 min) for relative quantification of these lipid isomers. The DMS-MS results show excellent agreement with established, but time-consuming, enzymatic approaches and also provide superior accuracy to methods that rely on MS alone. The advantages of this DMS-MS method in identification and quantification of GP isomer populations is demonstrated by direct analysis of complex biological extracts without any prior fractionation.

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ID Code: 79550
Item Type: Journal Article
Refereed: Yes
DOI: 10.1194/jlr.M046995
ISSN: 1539-7262
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100) > Analytical Spectrometry (030101)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
Copyright Owner: Copyright 2014 American Society for Biochemistry and Molecular Biology
Copyright Statement: This research was originally published in Journal of Lipid Research. Maccarone, Alan T., Duldig, Jackson, Mitchell, Todd W., Blanksby, Stephen J., Duchoslav, Eva, & Campbell, J. Larry. Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry. Journal of Lipid Research. 2014. 55, 1668-1677. © the American Society for Biochemistry and Molecular Biology
Deposited On: 21 Dec 2014 23:34
Last Modified: 17 Sep 2015 05:35

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