Computational epigenetic profiling of CpG islets in MTHFR

Wei, Keat, Sutherland, Heidi, Camilleri, Emily, Haupt, Larisa M., Griffiths, Lyn R., & Gan, Siew Hua (2014) Computational epigenetic profiling of CpG islets in MTHFR. Molecular Biology Reports, 41(12), pp. 8285-8292.

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Abstract

Computational epigenetics is a new area of research focused on exploring how DNA methylation patterns affect transcription factor binding that affect gene expression patterns. The aim of this study was to produce a new protocol for the detection of DNA methylation patterns using computational analysis which can be further confirmed by bisulfite PCR with serial pyrosequencing. The upstream regulatory element and pre-initiation complex relative to CpG islets within the methylenetetrahydrofolate reductase gene were determined via computational analysis and online databases. The 1,104 bp long CpG island located near to or at the alternative promoter site of methylenetetrahydrofolate reductase gene was identified. The CpG plot indicated that CpG islets A and B, within the island, contained 62 and 75 % GC content CpG ratios of 0.70 and 0.80–0.95, respectively. Further exploration of the CpG islets A and B indicates that the transcription start sites were GGC which were absent from the TATA boxes. In addition, although six PROSITE motifs were identified in CpG B, no motifs were detected in CpG A. A number of cis-regulatory elements were found in different regions within the CpGs A and B. Transcription factors were predicted to bind to CpGs A and B with varying affinities depending on the DNA methylation status. In addition, transcription factor binding may influence the expression patterns of the methylenetetrahydrofolate reductase gene by recruiting chromatin condensation inducing factors. These results have significant implications for the understanding of the architecture of transcription factor binding at CpG islets as well as DNA methylation patterns that affect chromatin structure.

Impact and interest:

3 citations in Scopus
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2 citations in Web of Science®

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ID Code: 79632
Item Type: Journal Article
Refereed: Yes
Keywords: MTHFR, Epigenetics, Computational, CpG, Transcription factor
DOI: 10.1007/s11033-014-3729-x
ISSN: 1573-4978
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 Springer Science+Business Media Dordrecht
Deposited On: 23 Dec 2014 04:10
Last Modified: 04 Mar 2015 00:57

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