FT-Raman and FT-IR spectroscopic study of the local structure of synthetic Mg/Zn/Al-hydrotalcites
Kloprogge, J. Theo, Frost, Ray L., & Hickey, Leisel (2004) FT-Raman and FT-IR spectroscopic study of the local structure of synthetic Mg/Zn/Al-hydrotalcites. Journal of Raman Spectroscopy, 35, pp. 967-974.
Nitriles introduced into peptides and proteins can serve as useful vibrational spectroscopic probes, because the nitrile C ≡ N stretch is well isolated from backbone and sidechain vibrational bands. Aromatic nitriles offer large νC ≡ N absorption intensity in infrared spectra and resonance enhancement in Raman spectra with ultraviolet excitation. We report the ultraviolet resonance Raman spectra of cyanophenylalanine attached to cysteine, through linkage reactions that are applicable to cysteine residues in proteins. Excitation profiles are reported, and the νC ≡ N detection limit is estimated to be 5 µ m. The band position is sensitive to solvent polarity and especially to strong H-bonding. The derivatization of mastoparan X peptide at introduced cysteine residues demonstrated the effectiveness of a cyanophenylcysteine probe in reporting the lowered environmental polarity when the peptide was incorporated into liposomes. For an asymmetrical cyanophenyl derivative, 2-CBCys, the intensity ratio of asymmetric and symmetric ring modes, ν8b and ν8a, was found to respond to solvent polarity and not to H-bonding.
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|Item Type:||Journal Article|
|Keywords:||FT, IR spectroscopy, FT, Raman, hydrotalcite, hydroxycarbonate, layered double hydroxide, LDH, synthesis|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > INORGANIC CHEMISTRY (030200)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
Past > Schools > School of Physical & Chemical Sciences
|Copyright Owner:||Copyright 2004 John Wiley & Sons|
|Copyright Statement:||The definite version is available on publication at www3.interscience.wiley.com|
|Deposited On:||09 Mar 2005|
|Last Modified:||02 Jul 2013 09:53|
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