Review-evaluating the molecular assays for measuring the oxidative potential of particulate matter

Hedayat, F., Stevanovic, S., Miljevic, B., Bottle, S., & Ristovski, Z.D. (2014) Review-evaluating the molecular assays for measuring the oxidative potential of particulate matter. Chemical Industry and Chemical Engineering Quarterly, 21(1-2), pp. 201-210.

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Several cell-free assays are currently used to quantify and detect the Reactive Oxygen Species (ROS). All of them have certain limitations, do not provide direct comparison of results and, to date, none of these assays have been acknowledged as the most suitable acellular assay and none has yet been adopted for investigation of potential PM toxicity. These assays include DTT, ascorbic acid, DCFHDA and PFN assays which have been used in measurements of the particles generated from various combustion sources such as diesel engine, wood smoke (or biomass burning) and cigarette smoke, as well as for outdoor measurements. All the probes use different units for expressing redox properties of PM. Also, their reactivity is being triggered by different types of ROS. This limits the direct comparison of the results that are reporting the toxicity of the same aerosol type measured with various probes. This study is evaluating and comparing the various assays in order to develop deeper understanding of their capabilities, selectivity as well as improve understanding of the underlying chemical mechanisms.

Keywords: DTT, DCFH-DA, PFN, BPEA-nit, Ascorbic acid, oxidative potential

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8 citations in Scopus
7 citations in Web of Science®
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ID Code: 79807
Item Type: Journal Article
Refereed: Yes
DOI: 10.2298/ciceq140228031h
ISSN: 2217-7434
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 06 Jan 2015 03:26
Last Modified: 29 Apr 2015 12:26

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