Transcriptome analyses of amoebic gill disease-affected atlantic salmon (Salmo salar) tissues reveal localized host gene suppression

Wynne, J. W., O'Sullivan, M. G., Cook, M. T., Stone, G., Nowak, B. F., Lovell, D. R., & Elliott, N. G. (2008) Transcriptome analyses of amoebic gill disease-affected atlantic salmon (Salmo salar) tissues reveal localized host gene suppression. Marine Biotechnology, 10(4), pp. 388-403.

View at publisher

Abstract

The transcriptome response of Atlantic salmon (Salmo salar) displaying advanced stages of amoebic gill disease (AGD) was investigated. Naïve smolt were challenged with AGD for 19 days, at which time all fish were euthanized and their severity of infection quantified through histopathological scoring. Gene expression profiles were compared between heavily infected and naïve individuals using a 17 K Atlantic salmon cDNA microarray with real-time quantitative RT-PCR (qPCR) verification. Expression profiles were examined in the gill, anterior kidney, and liver. Twenty-seven transcripts were significantly differentially expressed within the gill; 20 of these transcripts were down-regulated in the AGD-affected individuals compared with naïve individuals. In contrast, only nine transcripts were significantly differentially expressed within the anterior kidney and five within the liver. Again the majority of these transcripts were down-regulated within the diseased individuals. A down-regulation of transcripts involved in apoptosis (procathepsin L, cathepsin H precursor, and cystatin B) was observed in AGD-affected Atlantic salmon. Four transcripts encoding genes with antioxidant properties also were down-regulated in AGD-affected gill tissue according to qPCR analysis. The most up-regulated transcript within the gill was an unknown expressed sequence tag (EST) whose expression was 218-fold (± SE 66) higher within the AGD affected gill tissue. Our results suggest that Atlantic salmon experiencing advanced stages of AGD demonstrate general down-regulation of gene expression, which is most pronounced within the gill. We propose that this general gene suppression is parasite-mediated, thus allowing the parasite to withstand or ameliorate the host response. © 2008 Springer Science+Business Media, LLC.

Impact and interest:

15 citations in Scopus
Search Google Scholar™
31 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 79856
Item Type: Journal Article
Refereed: Yes
Keywords: AGD, Host response, Microarray, Parasite infection, Salmonid, Ameliorate, Amoebic gill disease (AGD), QPCR analysis, Salmonids, Disease control, DNA, Genetic engineering, Marine biology, Real time control, Transcription, Tissue engineering, gene expression, parasite infestation, parasitic disease, parasitism, polymerase chain reaction, Amoeba, animal, animal disease, article, Atlantic salmon, DNA microarray, fish disease, gene expression profiling, gene expression regulation, genetics, gill, kidney, liver, metabolism, parasitology, pathology, physiology, reproducibility, sarcomastigophora infection, Amoebida, Animals, Fish Diseases, Gills, Oligonucleotide Array Sequence Analysis, Reproducibility of Results, Salmo salar, Sarcomastigophora Infections, Salmonidae
DOI: 10.1007/s10126-007-9075-4
ISSN: 1436-2228
Subjects: Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000)
Divisions: Current > Schools > School of Electrical Engineering & Computer Science
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Springer
Deposited On: 07 Jan 2015 04:22
Last Modified: 21 Jan 2015 04:01

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page