Unlocking Hidden Genomic Sequence
Despite the success of conventional Sanger
sequencing, signi®cant regions of many genomes
still present major obstacles to sequencing. Here we
propose a novel approach with the potential to alleviate
a wide range of sequencing dif®culties. The
technique involves extracting target DNA sequence
from variants generated by introduction of random
mutations. The introduction of mutations does not
destroy original sequence information, but distributes
it amongst multiple variants. Some of these
variants lack problematic features of the target and
are more amenable to conventional sequencing.
The technique has been successfully demonstrated
with mutation levels up to an average 18% base substitution
and has been used to read previously
intractable poly(A), AT-rich and GC-rich motifs.
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|Item Type:||Journal Article|
|Subjects:||Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2004 Oxford University Press|
|Copyright Statement:||This is an electronic version of an article published in [Nucleic Acids Research 32(3):e35]|
|Deposited On:||06 Jun 2007|
|Last Modified:||29 Feb 2012 23:29|
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