Unlocking hidden genomic sequence

Keith, Jonathan M., Cochran, Duncan A., Lala, Gita H., Adams, Peter, Bryant, Darryn, & Mitchelson, Keith R. (2004) Unlocking hidden genomic sequence. Nucleic Acids Research, 32(3), e35.

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Abstract

Despite the success of conventional Sanger sequencing, significant regions of many genomes still present major obstacles to sequencing. Here we propose a novel approach with the potential to alleviate a wide range of sequencing difficulties. The technique involves extracting target DNA sequence from variants generated by introduction of random mutations. The introduction of mutations does not destroy original sequence information, but distributes it amongst multiple variants. Some of these variants lack problematic features of the target and are more amenable to conventional sequencing. The technique has been successfully demonstrated with mutation levels up to an average 18% base substitution and has been used to read previously intractable poly(A), AT-rich and GC-rich motifs.

Impact and interest:

16 citations in Scopus
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13 citations in Web of Science®

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Full-text downloads:

145 since deposited on 06 Jun 2007
14 in the past twelve months

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ID Code: 8016
Item Type: Journal Article
Additional Information: Articles free to read on journal website
DOI: 10.1093/nar/gnh022
ISSN: 0305-1048
Subjects: Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2004 Oxford University Press
Copyright Statement: This is an electronic version of an article published in [Nucleic Acids Research 32(3):e35]
Deposited On: 06 Jun 2007
Last Modified: 29 Jan 2015 01:37

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