Unlocking hidden genomic sequence
Despite the success of conventional Sanger sequencing, significant regions of many genomes still present major obstacles to sequencing. Here we propose a novel approach with the potential to alleviate a wide range of sequencing difficulties. The technique involves extracting target DNA sequence from variants generated by introduction of random mutations. The introduction of mutations does not destroy original sequence information, but distributes it amongst multiple variants. Some of these variants lack problematic features of the target and are more amenable to conventional sequencing. The technique has been successfully demonstrated with mutation levels up to an average 18% base substitution and has been used to read previously intractable poly(A), AT-rich and GC-rich motifs.
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|Item Type:||Journal Article|
|Additional Information:||Articles free to read on journal website|
|Subjects:||Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2004 Oxford University Press|
|Copyright Statement:||This is an electronic version of an article published in [Nucleic Acids Research 32(3):e35]|
|Deposited On:||06 Jun 2007 00:00|
|Last Modified:||29 Jan 2015 01:37|
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