Chondrocyte redifferentiation and construct mechanical property development in single-component photocrosslinkable hydrogels

Levett, Peter, Melchels, Ferry, Schrobback, Karsten, Hutmacher, Dietmar, Malda, Jos, & Klein, Travis (2014) Chondrocyte redifferentiation and construct mechanical property development in single-component photocrosslinkable hydrogels. Journal of Biomedical Materials Research - Part A, 102(8), pp. 2544-2553.

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Hydrogels are promising materials for cartilage repair, but the properties required for optimal functional outcomes are not yet known. In this study, we functionalized four materials that are commonly used in cartilage tissue engineering and evaluated them using in vitro cultures. Gelatin, hyaluronic acid, polyethylene glycol, and alginate were functionalized with methacrylic anhydride to make them photocrosslinkable. We found that the responses of encapsulated human chondrocytes were highly dependent on hydrogel type. Gelatin hydrogels supported cell proliferation and the deposition of a glycosaminoglycan rich matrix with significant mechanical functionality. However, cells had a dedifferentiated phenotype, with high expression of collagen type I. Chondrocytes showed the best redifferentiation in hyaluronic acid hydrogels, but the newly formed matrix was highly localized to the pericellular regions, and these gels degraded rapidly. Polyethylene glycol hydrogels, as a bioinert control, did not promote any strong responses. Alginate hydrogels did not support the deposition of new matrix, and the stiffness decreased during culture. The markedly different response of chondrocytes to these four photocrosslinkable hydrogels demonstrates the importance of material properties for chondrogenesis and extracellular matrix production, which are critical for effective cartilage repair.

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12 citations in Web of Science®

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ID Code: 80534
Item Type: Journal Article
Refereed: Yes
Keywords: cartilage;hydrogels;photopolymerization;chondrogenesis;tissue engineering
DOI: 10.1002/jbm.a.34924
ISSN: 1549-3296
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Wiley
Copyright Statement: © 2013 Wiley Periodicals,
Deposited On: 21 Jan 2015 22:48
Last Modified: 30 Mar 2015 03:10

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