Activation of the canonical Wnt signaling pathway induces cementum regeneration

Han, Pingping, Ivanovski, Saso, Crawford, Ross, & Xiao, Yin (2015) Activation of the canonical Wnt signaling pathway induces cementum regeneration. Journal of Bone and Mineral Research, 30(7), pp. 1160-1174.

View at publisher


Canonical Wnt signaling is important in tooth development but it is unclear whether it can induce cementogenesis and promote the regeneration of periodontal tissues lost due to disease. Therefore, the aim of this study is to investigate the influence of canonical Wnt signaling enhancers on human periodontal ligament cell (hPDLCs) cementogenic differentiation in vitro and cementum repair in a rat periodontal defect model. Canonical Wnt signaling was induced by (i) local injection of lithium chloride; (ii) local injection of sclerostin antibody; and (iii) local injection of a lentiviral construct overexpressing β-catenin. The results showed that the local activation of canonical Wnt signaling resulted in significant new cellular cementum deposition and the formation of well-organized periodontal ligament fibers, which was absent in the control group. In vitro experiments using hPDLCs showed that the Wnt signaling pathway activators significantly increased mineralization, alkaline phosphatase (ALP) activity, and gene and protein expression of the bone and cementum markers osteocalcin (OCN), osteopontin (OPN), cementum protein 1 (CEMP1), and cementum attachment protein (CAP). Our results show that the activation of the canonical Wnt signaling pathway can induce in vivo cementum regeneration and in vitro cementogenic differentiation of hPDLCs.

Impact and interest:

5 citations in Scopus
Search Google Scholar™
3 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

3 since deposited on 24 Jun 2015
1 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 80825
Item Type: Journal Article
Refereed: Yes
Keywords: the canonical Wnt signaling pathway, regenerative medicine, cementum regeneration, tissue engineering
DOI: 10.1002/jbmr.2445
ISSN: 1523-4681
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 American Society for Bone and Mineral Research
Copyright Statement: This is the accepted version of the following article: Han, P., Ivanovski, S., Crawford, R. and Xiao, Y. (2015), Activation of the Canonical Wnt Signaling Pathway Induces Cementum Regeneration. J Bone Miner Res, 30: 1160–1174. doi: 10.1002/jbmr.2445, which has been published in final form at
Deposited On: 24 Jun 2015 00:16
Last Modified: 09 Aug 2016 22:33

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page