Activation of the canonical Wnt signaling pathway induces cementum regeneration
Han, Pingping, Ivanovski, Saso, Crawford, Ross, & Xiao, Yin (2015) Activation of the canonical Wnt signaling pathway induces cementum regeneration. Journal of Bone and Mineral Research, 30(7), pp. 1160-1174.
Canonical Wnt signaling is important in tooth development but it is unclear whether it can induce cementogenesis and promote the regeneration of periodontal tissues lost due to disease. Therefore, the aim of this study is to investigate the influence of canonical Wnt signaling enhancers on human periodontal ligament cell (hPDLCs) cementogenic differentiation in vitro and cementum repair in a rat periodontal defect model. Canonical Wnt signaling was induced by (i) local injection of lithium chloride; (ii) local injection of sclerostin antibody; and (iii) local injection of a lentiviral construct overexpressing β-catenin. The results showed that the local activation of canonical Wnt signaling resulted in significant new cellular cementum deposition and the formation of well-organized periodontal ligament fibers, which was absent in the control group. In vitro experiments using hPDLCs showed that the Wnt signaling pathway activators significantly increased mineralization, alkaline phosphatase (ALP) activity, and gene and protein expression of the bone and cementum markers osteocalcin (OCN), osteopontin (OPN), cementum protein 1 (CEMP1), and cementum attachment protein (CAP). Our results show that the activation of the canonical Wnt signaling pathway can induce in vivo cementum regeneration and in vitro cementogenic differentiation of hPDLCs.
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|Item Type:||Journal Article|
|Keywords:||the canonical Wnt signaling pathway, regenerative medicine, cementum regeneration, tissue engineering|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)|
|Divisions:||Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2015 American Society for Bone and Mineral Research|
|Copyright Statement:||This is the accepted version of the following article: Han, P., Ivanovski, S., Crawford, R. and Xiao, Y. (2015), Activation of the Canonical Wnt Signaling Pathway Induces Cementum Regeneration. J Bone Miner Res, 30: 1160–1174. doi: 10.1002/jbmr.2445, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbmr.2445/abstract|
|Deposited On:||24 Jun 2015 00:16|
|Last Modified:||09 Aug 2016 22:33|
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