N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Ho, Jenny, Al-Deen, Fatin M. Nawwab, Al-Abboodi, Aswan, Selomulya, Cordelia, Xiang, Sue D., Plebanski, Magdalena, & Forde, Gareth M. (2011) N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier. Colloids and Surfaces B : Biointerfaces, 83(1), pp. 83-90.

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Abstract

Particulates with specific sizes and characteristics can induce potent immune responses by promoting antigen uptake of appropriate immuno-stimulatory cell types. Magnetite (Fe3O4) nanoparticles have shown many potential bioapplications due to their biocompatibility and special characteristics. Here, superparamagnetic Fe3O4 nanoparticles (SPIONs) with high magnetization value (70emug-1) were stabilized with trisodium citrate and successfully conjugated with a model antigen (ovalbumin, OVA) via N,N'-carbonyldiimidazole (CDI) mediated reaction, to achieve a maximum conjugation capacity at approximately 13μgμm-2. It was shown that different mechanisms governed the interactions between the OVA molecules and magnetite nanoparticles at different pH conditions. We evaluated as-synthesized SPION against commercially available magnetite nanoparticles. The cytotoxicity of these nanoparticles was investigated using mammalian cells. The reported CDI-mediated reaction can be considered as a potential approach in conjugating biomolecules onto magnetite or other biodegradable nanoparticles for vaccine delivery.

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ID Code: 81527
Item Type: Journal Article
Refereed: Yes
Keywords: Magnetite, N, N'-Carbonyldiimidazole, Particulate vaccine carrier, Superparamagnetic nanoparticles, Bioapplications, Biodegradable nanoparticle, Carbonyldiimidazole, Cell types, Different mechanisms, Functionalizations, High magnetization, Immune response, Magnetite nanoparticles, Mammalian cells, Mediated reactions, Ovalbumins, pH condition, Superparamagnetics, Trisodium citrates, Vaccine carriers, Vaccine delivery, Antigens, Biocompatibility, Mammals, Superparamagnetism, Vaccines, Nanoparticles, citrate trisodium, drug carrier, imidazole derivative, ovalbumin, superparamagnetic iron oxide nanoparticle, unclassified drug, vaccine, adsorption kinetics, animal cell, article, cell viability, controlled study, cytotoxicity test, drug cytotoxicity, drug delivery system, hydrodynamics, hydrophilicity, isoelectric point, isotherm, light scattering, magnetic field, molecular interaction, molecular weight, nonhuman, particle size, pH, priority journal, scanning electron microscopy, transmission electron microscopy, zeta potential, Animals, Cell Death, Cell Survival, Cercopithecus aethiops, COS Cells, Drug Carriers, Electrophoresis, Polyacrylamide Gel, Ferric Compounds, Hydrogen-Ion Concentration, Imidazoles, Magnetics, Spectroscopy, Fourier Transform Infrared, Temperature, X-Ray Diffraction, Mammalia
DOI: 10.1016/j.colsurfb.2010.11.001
ISSN: 0927-7765
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Feb 2015 23:38
Last Modified: 11 Feb 2015 01:17

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