Microparticle-mediated gene delivery for the enhanced expression of a 19-KDa fragment of merozoite surface protein 1 of Plasmodium falciparum

Liu, Shan, Danquah, Michael K., Forde, Gareth M., Ma, Charles, Wang, Lina, & Coppel, Ross (2010) Microparticle-mediated gene delivery for the enhanced expression of a 19-KDa fragment of merozoite surface protein 1 of Plasmodium falciparum. Biotechnology Progress, 26(1), pp. 257-262.

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Abstract

The 19 kDa carboxyl-terminal fragment of merozoite surface protein 1 (MSP119) is a major component of the invasion-inhibitory response in individual immunity to malaria. A novel ultrasonic atomization approach for the formulation of biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles of malaria DNA vaccines encoding MSP119 is presented here. After condensing the plasmid DNA (pDNA) molecules with a cationic polymer polyethylenimine (PEI), a 40 kHz ultrasonic atomization frequency was used to formulate PLGA microparticles at a flow rate of 18 mL h1. High levels of gene expression and moderate cytotoxicity in COS-7 cells were achieved with the condensed pDNA at a nitrogen to phosphate (N/P) ratio of 20, thus demonstrating enhanced cellular uptake and expression of the transgene. The ability of the microparticles to convey pDNA was examined by characterizing the formulated microparticles. The microparticles displayed Z-average hydrodynamic diameters of 1.50-2.10 lm and zeta potentials of 17.8-23.2 mV. The encapsulation efficiencies were between 78 and 83%, and 76 and 85% of the embedded malaria pDNA molecules were released under physiological conditions in vitro. These results indicate that PLGA-mediated microparticles can be employed as potential gene delivery systems to antigen-presenting cells in the prevention of malaria.

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4 citations in Scopus
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3 citations in Web of Science®

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ID Code: 81532
Item Type: Journal Article
Refereed: Yes
Keywords: DNA vaccine delivery, Microparticles, Plasmid DNA, Poly(lactic-co-glycolic acid), Ultrasonic atomization, DNA vaccine, Micro-particles, Acids, Atomization, DNA, Encapsulation, Gene transfer, Jets, Microbiology, Proteins, Ultrasonics, Vaccines, Zeta potential, Gene expression, lactic acid, malaria vaccine, merozoite surface protein 1, polyethyleneimine, polyglycolic acid, polylactic acid polyglycolic acid copolymer, polylactic acid-polyglycolic acid copolymer, animal, article, cell strain COS1, cell survival, Cercopithecus, chemistry, drug effect, genetics, immunology, particle size, Plasmodium falciparum, structure activity relation, Animals, Cercopithecus aethiops, COS Cells, Gene Transfer Techniques, Malaria Vaccines, Structure-Activity Relationship, Vaccines, DNA
DOI: 10.1002/btpr.318
ISSN: 8756-7938
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Feb 2015 23:40
Last Modified: 11 Feb 2015 02:01

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