Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Roberts, Michael W. H., Ongkudon, Clarence M., Forde, Gareth M., & Danquah, Michael K. (2009) Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules. Journal of Separation Science, 32(15-16), pp. 2485-2494.

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Polymethacrylate monoliths, specifically poly(glycidyl methacrylate-co-ethylene dimethacrylate) or poly(GMA-co-EDMA) monoliths, are a new generation of chromatographic supports and are significantly different from conventional particle-based adsorbents, membranes, and other monolithic supports for biomolecule purification. Similar to other monoliths, polymethacrylate monoliths possess large pores which allow convective flow of mobile phase and result in high flow rates at reduced pressure drop, unlike particulate supports. The simplicity of the adsorbent synthesis, pH resistance, and the ease and flexibility of tailoring their pore size to that of the target biomolecule are the key properties which differentiate polymethacrylate monoliths from other monoliths. Polymethacrylate monoliths are endowed with reactive epoxy groups for easy functionalization (with anion-exchange, hydrophobic, and affinity ligands) and high ligand retention. In this review, the structure and performance of polymethacrylate monoliths for chromatographic purification of biomolecules are evaluated and compared to those of other supports. The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through-put biomolecule purification on semi-preparative and preparative scales.

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23 citations in Web of Science®

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ID Code: 81538
Item Type: Journal Article
Refereed: Yes
Keywords: Biomolecules, Chromatography, Polymethacrylate monolith, Purification, Affinity ligands, Anion exchange, Chromatographic purification, Chromatographic supports, Convective flow, Epoxy group, Ethylene dimethacrylate, Functionalizations, Glycidyl methacrylate, High throughput, High-flow rate, Large pores, Mobile phase, Monolithic supports, pH resistance, Polymethacrylates, Reduced pressure, Structure and performance, Acrylic monomers, Adsorbents, Adsorption, Chromatographic analysis, Ethylene, Fluorine containing polymers, Ligands, Monolithic integrated circuits, manganese peroxidase, nucleic acid, oligonucleotide, poly(glycidyl methacrylate co ethylene dimethacrylate), polymethacrylic acid, unclassified drug, peptide, polymethacrylic acid derivative, protein, anion exchange chromatography, chemical structure, column chromatography, flow rate, hydrodynamics, hydrophobicity, monolithic chromatography, particle size, pH, polymerization, porosity, priority journal, review, separation technique, virus particle, chemistry, instrumentation, isolation and purification, mathematics, methodology, pressure, surface property, virion, Nucleic Acids, Oligonucleotides, Peptides, Polymethacrylic Acids, Proteins, Surface Properties
DOI: 10.1002/jssc.200900309
ISSN: 1615-9306
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2009 Wiley - V C H Verlag GmbH & Co. KGaA
Deposited On: 05 Feb 2015 23:28
Last Modified: 11 Feb 2015 02:55

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