Preparation and characterization of poly(lactic-co-glycolic acid) microparticles containing DNA molecules encoding a malaria vaccine candidate

Liu, Shan, Danquah, Michael K., Ho, Jenny, Ma, Charles, Wang, Lina, Coppel, Ross, & Forde, Gareth M. (2009) Preparation and characterization of poly(lactic-co-glycolic acid) microparticles containing DNA molecules encoding a malaria vaccine candidate. Journal of Chemcial Technology and Biotechnology, 84(5), pp. 782-788.

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Abstract

Background

A novel ultrasonic atomization approach for the formulation of biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles of a malaria DNA vaccine is presented. A 40 kHz ultrasonic atomization device was used to create the microparticles from a feedstock containing 5 volumes of 0.5% w/v PLGA in acetone and 1 volume of condensed DNA which was fed at a flow rate of 18ml h-1. The plasmid DNA vectors encoding a malaria protein were condensed with a cationic polymer before atomization.

Results

High levels of gene expression in vitro were observed in COS-7 cells transfected with condensed DNA at a nitrogen to phosphate (N/P) ratio of 10. At this N/P ratio, the condensed DNA exhibited a monodispersed nanoparticle size (Z-average diameter of 60.8 nm) and a highly positive zeta potential of 38.8mV. The microparticle formulations of malaria DNA vaccine were quality assessed and it was shown that themicroparticles displayed high encapsulation efficiencies between 82-96% and a narrow size distribution in the range of 0.8-1.9 μm. In vitro release profile revealed that approximately 82% of the DNA was released within 30 days via a predominantly diffusion controlledmass transfer system.

Conclusions

This ultrasonic atomization technique showed excellent particle size reproducibility and displayed potential as an industrially viable approach for the formulation of controlled release particles.

Impact and interest:

4 citations in Scopus
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3 citations in Web of Science®

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ID Code: 81539
Item Type: Journal Article
Refereed: Yes
Keywords: Enhanced transfection, Malaria DNA vaccine, Microparticles, UItrasonic atomization, Average diameter, Cationic polymers, Condensed DNA, Controlled release, DNA molecules, DNA vaccine, Encapsulation efficiency, In-vitro, Malaria vaccine, Monodispersed nanoparticles, Narrow size distributions, Plasmid DNA vector, Poly(lactic-co-glycolic acid), Reproducibility, Transfer systems, Ultrasonic atomization, Acetone, Acids, Atomization, DNA, Encapsulation, Encoding (symbols), Gene expression, Jets, Ultrasonic testing, Ultrasonics, Vaccines, Zeta potential, Nucleic acids, nanoparticle, plasmid DNA, polyglactin, animal cell, article, biodegradability, controlled release formulation, controlled study, flow rate, in vitro gene transfer, nebulization, nonhuman, particle size
DOI: 10.1002/jctb.2112
ISSN: 0268-2575
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Feb 2015 23:57
Last Modified: 11 Feb 2015 03:01

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