Single step purification of plasmid DNA using peptide ligand affinity chromatography

Han, Ying & Forde, Gareth M. (2008) Single step purification of plasmid DNA using peptide ligand affinity chromatography. Journal of Chromatography B : Analytical Technologies in the Biomedical and Life Sciences, 874(1-2), pp. 21-26.

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Single step affinity chromatography was employed for the purification of plasmid DNA (pDNA), thus eliminating several steps compared with current commercial purification methods for pDNA. Significant reduction in pDNA production time and cost was obtained. This chromatographic operation employed a peptide-monolith construct to isolate pDNA from Escherichia coli (E. coli) impurities present in a clarified lysate feedstock. Mild conditions were applied to avoid any degradation of pDNA. The effect of some important parameters on pDNA yield was also evaluated with the aim of optimising the affinity purification of pDNA. The results demonstrate that 81% of pDNA was recovered and contaminating gDNA, RNA and protein were removed below detectable levels. © 2008 Elsevier B.V. All rights reserved.

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ID Code: 81548
Item Type: Journal Article
Refereed: Yes
Keywords: Affinity chromatography, Ligand, Peptide, Plasmid DNA purification, Single step purification, Amines, Chromatographic analysis, Chromatography, Clarification, DNA, Escherichia coli, Genes, Nucleic acids, Organic acids, Raw materials, RNA, Water pollution, Purification, plasmid DNA, article, cell lysate, DNA degradation, DNA isolation, DNA purification, peptide analysis, priority journal, Chromatography, Affinity, Ligands, Peptides, Plasmids, Reproducibility of Results
DOI: 10.1016/j.jchromb.2008.08.025
ISSN: 1570-0232
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2008 Elsevier B.V.
Deposited On: 05 Feb 2015 23:06
Last Modified: 11 Feb 2015 03:46

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